Multiple choice

Which of the following statements regarding DNA footprinting procedure is incorrect?

  1. DNA fragment thought to contain protein binding sequence is extracted, amplified and labelled at both the ends of DNA.

  2. Protein has protected the DNA binding site from cleavage agent.

  3. DNA fragments are separated by polyacrylamide gel electrophoresis.

  4. The missing band indicates the protein binding specific DNA sequence.

  5. The labelled DNA fragments are mixed with cleavage agents without DNA binding protein.
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A Correct answer
Explanation

This statement is incorrect, hence the right answer. DNA fragment thought to contain protein binding sequence is extracted, amplified and labelled at one end of the double helix using polymerase chain reaction technique.

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