Multiple choice

What is the correct sequence in DNA fingerprinting?

  1. (1) Isolation of DNA (2) Digestion of DNA by restriction endonucleases (3) Separation of DNA fragments by electrophoresis (4) Transferring (blotting) of separated DNA fragments to synthetic membranes (5) Detection of hybridised DNA fragments by autoradiography (6) Hybridisation using labelled VNTR probe

  2. (1) Isolation of DNA (2) Separation of DNA fragments by electrophoresis (3) Digestion of DNA by restriction endonucleases (4) Transferring (blotting) of separated DNA fragments (5) Hybridisation using labelled VNTR probe (6) Detection of hybridised DNA fragments by autoradiography

  3. (1) Digestion of DNA by restriction endonucleases (2) Isolation of DNA (3) Transferring (blotting) of separated DNA fragments to synthetic membranes (4) Separation of DNA fragments by electrophoresis (5) Hybridisation using labelled VNTR probe (6) Detection of hybridised DNA fragments by autoradiography

  4. (1) Isolation of DNA (2) Digestion of DNA by restriction endonucleases (3) Separation of DNA fragments by electrophoresis (4) Transferring (blotting) of separated DNA fragments to synthetic membranes (5) Hybridisation using labelled VNTR probe (6) Detection of hybridised DNA fragments by autoradiography

  5. (1) Isolation of DNA (2) Digestion of DNA by restriction endonucleases (3) Transferring (blotting) of separated DNA fragments to synthetic membranes (4) Separation of DNA fragments by electrophoresis (5) Hybridisation using labelled VNTR probe (6) Detection of hybridised DNA fragments by autoradiography
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D Correct answer
Explanation

The correct sequence in DNA fingerprinting is: (1) Isolation of DNA (2) Digestion of DNA by restriction endonucleases (3) Separation of DNA fragments by electrophoresis (4) Transferring (blotting) of separated DNA fragments to synthetic membranes (5) Hybridisation using labelled VNTR probe (6) Detection of hybridised DNA fragments by autoradiography

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