Multiple choice

Different steps of Variable Number of Tandem Repeats (VNTR) technique are given below: (i) Isolation of DNA, (ii) Separation of DNA fragments by electrophoresis (iii) Digestion of DNA by restriction endonucleases (iv) Hybridisation using labelled VNTR probe (v) Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon (vi) Detection of hybridised DNA fragments by autoradiography.

The first and the last steps are placed correctly. Which one of the following arrangements of steps (ii) to (v) is correct?

  1. (ii) → (iii) → (iv) → (v)

  2. (iii) → (ii) → (iv) → (v)

  3. (iii) → (ii) → (v) → (iv)

  4. (ii) → (iii) → (v) → (iv)

  5. (ii) → (iv) → (iii) → (v)
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C Correct answer
Explanation

Correct order of various steps of Variable Number of Tandem Repeats (VNTR) technique is: (i) → (iii) → (ii) → (v) → (iv) → (vi) Isolation of DNA → digestion of DNA by restriction endonucleases → separation of DNA fragments by electrophoresis → transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon → hybridisation using labelled VNTR probe → detection of hybridised DNA fragments by autoradiography.

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