Home › Quizzes › Biotechnology and its Applications

Biotechnology and its Applications

Practice MCQ for Medical entrance examination on the topic Biotechnology and its application.

25 Questions Published
Play Quiz Practise this topic out loud

Questions

Question 1 Multiple Choice (Single Answer)

Which of the following organisms produces single cell protein?

  1. Geledium
  2. Methylophilus
  3. Methanobacterium
  4. Spirulina
  5. Gracilaria
Question 2 Multiple Choice (Single Answer)

Which of the following are the chemical knives used in rDNA technology?

  1. Reverse transcriptases
  2. Nucleases
  3. Polymerases
  4. Restriction endonucleases
  5. Ligases
Question 3 Multiple Choice (Single Answer)

Which of the following represents the genetic variation present among plants in a tissue culture?

  1. Heterosis
  2. Genetic drift
  3. Hybrid vigour
  4. Mutation
  5. Somaclonal variation
Question 4 Multiple Choice (Single Answer)

Which of the following is the first hormone produced artificially by bacteria and is widely employed as medicine?

  1. Adrenalin
  2. Thyroxine
  3. Testosterone
  4. Human insulin
  5. Antitrypsin
Question 5 Multiple Choice (Single Answer)

Which of the following is a thermostable enzyme?

  1. RNA polymerase
  2. Hind III
  3. DNA ligase
  4. Taq DNA polymerase
  5. EcoRl
Question 6 Multiple Choice (Single Answer)

In bacteria, the genes which confer antibiotic resistance on the bacteria are located on __________.

  1. mesosome
  2. m-RNA
  3. plasmid
  4. circular DNA molecule
  5. polysome
Question 7 Multiple Choice (Single Answer)

Which of the following organisms is known as natural genetic engineer?

  1. Escherichia coIi
  2. Bacillus thuringiensis
  3. Neurospora crassa
  4. Agrobacterium tumefaciens
  5. Rhizobium
Question 8 Multiple Choice (Single Answer)

The enzyme Reverse Transcriptase used in rDNA technology is extracted from

  1. T-phage
  2. Bacillus thuringiensis
  3. Avian Myeloblastosis virus
  4. Escherichia coli
  5. Neurospora
Question 9 Multiple Choice (Single Answer)

Which of the following enzymes are essential in protoplast fusion technique?

  1. Lipase and pectinase
  2. Amylase and pectinase
  3. Cellulase and pectinase
  4. Amylase and lipase
  5. Protease and nuclease
Question 10 Multiple Choice (Single Answer)

For the treatment of which of the following diseases was the first clinical gene therapy done?

  1. AIDS
  2. Cancer
  3. Myotonic dystrophy
  4. SCID
  5. Cystic fibrosis
Question 11 Multiple Choice (Single Answer)

Which of the following best describes 'pharming'?

  1. Large-scale production of cloned animals
  2. Animals being used in transgenic research
  3. Synthesis of a drug by a transgenic animal
  4. Synthesis of recombinant drugs by bacteria
  5. Plants making genetically-altered foods
Question 12 Multiple Choice (Single Answer)

The full form of GEAC is

  1. Genetic Engineering Approval Council
  2. Genetic Engineering Approval Commission
  3. Genomic Engineering Action Committee
  4. Genetic Engineering Approval Committee
  5. Genetic Engineering Action Committee
Question 13 Multiple Choice (Single Answer)

Emphysema is a dangerous lung disorder seen in smokers. The drug used to treat emphysema produced by genetic engineering is

  1. humulin
  2. statin
  3. cyclosporin A
  4. alpha 1 antitrypsin
  5. trypsin
Question 14 Multiple Choice (Single Answer)

Bt toxin is coded by a gene named cry. Which of the following genes encodes for the Bt toxin against corn borer?

  1. cryIAc
  2. cryIIAb
  3. cryIIIA
  4. cryIAb
  5. cryIIIB
Question 15 Multiple Choice (Single Answer)

B. thuringiensis forms protein crystals during a particular phase of their growth. These crystals contain a toxic insecticidal protein and exist as inactive protoxins, but once an insect ingests the inactive toxin, it is converted into an active form of toxin. This is due to __________.

  1. the acidic pH of the gut, which solubilises the crystals
  2. the neutral pH of the gut, which solubilises the crystals
  3. the alkaline pH of the gut, which solubilises the crystals
  4. the alkaline pH of the respiratory tract, which solubilises the crystals
  5. the acidic pH of the respiratory tract, which solubilises the crystals
Question 16 Multiple Choice (Single Answer)

A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield. It can be prevented by RNA interference (RNAi). What does this method involve?

  1. Silencing of a specific DNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
  2. Silencing of a specific tRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
  3. Silencing of a specific mRNA due to a complementary ssRNA molecule that binds to and prevents translation of the mRNA.
  4. Silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
  5. Silencing of a specific rRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
Question 17 Multiple Choice (Single Answer)

Identify the disorder caused due to ADA-Adenosine deaminase enzyme deficiency in human.

  1. Autoimmune disorders
  2. Acquired immune deficiency disorder
  3. Severe combined imuno deficiency
  4. Chronic lymphocytic leukemia
  5. Chronic myelocytic leukemia
Question 18 Multiple Choice (Single Answer)

Plants whose genes have been altered by manipulation are called genetically modified (GM) plants. GM plants have been useful in many ways. Which of the following is incorrect about GM plants?

  1. Enhanced nutritional value of food like golden rice
  2. Increased efficiency of mineral usage by plants to prevent early exhaustion of fertility of soil
  3. Helped to decrease post-harvest losses
  4. Made crops less tolerant to abiotic stresses like cold, drought, salt and heat
Question 19 Multiple Choice (Single Answer)

Golden rice is a transgenic rice in India. It has gene for synthesising _____________.

  1. tocopherol
  2. calciferol
  3. thiamine
  4. riboflavin
  5. beta carotene
Question 20 Multiple Choice (Single Answer)

Which probe is used in recombinant DNA technology and molecular diagnosis?

  1. A short, double or single-stranded DNA or RNA tagged with radioactive molecule to detect the product enzyme
  2. A short mRNA strand tagged with radioactive molecule to detect the codon sequence
  3. A short DNA strand tagged with radioactive molecule for identifying the coding and template strands
  4. A short, single-stranded DNA or RNA tagged with radioactive molecule to detect the complimentary strand
  5. A short, double DNA tagged with radioactive thimidine for recognising its corresponding complementary strand
Question 21 Multiple Choice (Single Answer)

Which of the following is a method commonly used in molecular biology for detection of a specific DNA sequence in DNA samples?

  1. Gel electrophoresis
  2. Northern blotting
  3. Western blotting
  4. Southern blotting
  5. Eastern blotting
Question 22 Multiple Choice (Single Answer)

From which of the following organisms are Ti plasmids isolated?

  1. Bacillus thuringiensis
  2. Rhizobium leguminosarum
  3. Agrobacterium tumefaciens
  4. Thermus aquaticus
  5. Escherichia coli
Question 23 Multiple Choice (Single Answer)

Which of the following enzymes is known as chemical suture?

  1. Restriction endonuclease
  2. Endonuclease
  3. Polymerase I
  4. Primase enzyme
  5. DNA-ligase
Question 24 Multiple Choice (Single Answer)

Match the column A showing restriction sites with column B showing restriction enzymes and find out correct match from the following.

 
Column - A Column - B
(i) 5' GAATTC 3' 
             3' CTTAAG 5'|(A) Sca I|

|(ii) 5' GGATCC 3'
3' CCTAGG 5'|(B) Sma I|
|(iii) 5' CCCGGG 3'
3' GGGCCC 5'|(C) Bam HI|
|(iv) 5' AGTACT 3'
3' TCATGA 5'|(D) EcoRI|

  1. 1 - A, 2 - B, 3 - C and 4 - D
  2. 1 - B, 2 - A, 3 - C and 4 - D
  3. 1 - C, 2 - B, 3 - A and 4 - D
  4. 1 - D, 2 - B, 3 - C and 4 - A
  5. 1 - D, 2 - C, 3 - B and 4 - A
Question 25 Multiple Choice (Single Answer)

What is the correct sequence in DNA fingerprinting?

  1. (1) Isolation of DNA
    (2) Digestion of DNA by restriction endonucleases
    (3) Separation of DNA fragments by electrophoresis
    (4) Transferring (blotting) of separated DNA fragments to synthetic membranes
    (5) Detection of hybridised DNA fragments by autoradiography
    (6) Hybridisation using labelled VNTR probe
  2. (1) Isolation of DNA
    (2) Separation of DNA fragments by electrophoresis
    (3) Digestion of DNA by restriction endonucleases
    (4) Transferring (blotting) of separated DNA fragments
    (5) Hybridisation using labelled VNTR probe
    (6) Detection of hybridised DNA fragments by autoradiography
  3. (1) Digestion of DNA by restriction endonucleases
    (2) Isolation of DNA
    (3) Transferring (blotting) of separated DNA fragments to synthetic membranes
    (4) Separation of DNA fragments by electrophoresis
    (5) Hybridisation using labelled VNTR probe
    (6) Detection of hybridised DNA fragments by autoradiography
  4. (1) Isolation of DNA
    (2) Digestion of DNA by restriction endonucleases
    (3) Separation of DNA fragments by electrophoresis
    (4) Transferring (blotting) of separated DNA fragments to synthetic membranes
    (5) Hybridisation using labelled VNTR probe
    (6) Detection of hybridised DNA fragments by autoradiography
  5. (1) Isolation of DNA
    (2) Digestion of DNA by restriction endonucleases
    (3) Transferring (blotting) of separated DNA fragments to synthetic membranes
    (4) Separation of DNA fragments by electrophoresis
    (5) Hybridisation using labelled VNTR probe
    (6) Detection of hybridised DNA fragments by autoradiography
← Back to Quizzes