Multiple choice

Match the following and choose the correct combination.

 
Group I Group II
A. blastx 1. Search protein database using translated nucleotide query
B. tblastn 2. Finding primers specific to your PCR template
C. tblastx 3. Search translated nucleotide database using a protein query
D. primer – BLAST 4. Search translated nucleotide database using a translated nucleotide query

  1. A - 3, B - 1, C - 2, D - 4

  2. A - 3, B - 1, C - 4, D - 2

  3. A - 1, B - 3, C - 4, D - 2

  4. A - 3, B - 4, C - 1, D - 2
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

blastx translates a nucleotide query in all six reading frames to search a protein database (A-1). tblastn uses a protein query to search a six-frame translation of a nucleotide database (B-3). tblastx performs six-frame translation on both query and database (C-4). Primer-BLAST is specifically designed for finding PCR primers unique to a template (D-2).

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