Multiple choice

Match the entries in group I with that in group II and select the correct answer.

 
Group I
Group II
P. Overlap-extension PCR 1. Splices together two or more DNA fragments that contain complementary sequences
Q. Hot start PCR 2. Reduces non-specific amplification during the initial set up stages of the PCR
R. Asymmetric PCR 3. Preferentially amplifies one DNA strand in a double-stranded DNA template
S. Digital PCR 4. Measures the quantity of a target DNA sequence in a DNA sample

  1. P - 1, Q - 2, R - 3, S - 4

  2. P - 1, Q - 3, R - 2, S - 4

  3. P - 2, Q - 1, R - 4, S - 3

  4. P - 2, Q - 3, R - 4, S - 1

  5. P - 4, Q - 3, R - 2, S - 1
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A Correct answer
Explanation

Overlap-extension PCR is a genetic engineering technique that is used to splice together two or more DNA fragments that contain complementary sequences. Hot start PCR is a technique that reduces non-specific amplification during the initial set up stages of the PCR. Asymmetric PCR preferentially amplifies one DNA strand in a double-stranded DNA template. Digital PCR is used to measure the quantity of a target DNA sequence in a DNA sample.

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