Multiple choice

The PCR (polymerase chain reaction) performed on a pool of long oligonucleotides with short overlapping segments is:

  1. intersequence-specific PCR

  2. inverse PCR

  3. hot Start PCR

  4. assembly PCR

  5. helicase-dependent amplification
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

The oligonucleotides alternate between sense and antisense directions, and the overlapping segments determine the order of the PCR fragments, thereby selectively producing the final long DNA product.

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