Multiple choice

The first step in the polymerase chain reaction (PCR) is

  1. denaturation

  2. heating

  3. primer extension

  4. annealing

  5. cooling

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A Correct answer
Explanation

Denaturation or separation of DNA strands is the first step. This step separates the strands by heating to a high temperature. Each separate strand can then serve as a template for copying.