Voges–Proskauer test is used to detect acetoin in bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth, which has been inoculated with bacteria. A cherry red colour indicates a positive result, while a yellow-brown color indicates a negative result.
Both Enterobacter aerogenes and Klebsiella pneumoniae give positive Voges–Proskauer test.