Multiple choice

Which of the following tests is used to detect acetoin in a bacterial broth culture?

  1. Citrate test

  2. Indole test

  3. Methyl red test

  4. Rapid urease test

  5. Voges-Proskauer test

Reveal answer Fill a bubble to check yourself
E Correct answer
Explanation

Voges–Proskauer test is used to detect acetoin in bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red colour indicates a positive result, while a yellow-brown colour indicates a negative result.