Which of the following tests is used to detect acetoin in a bacterial broth culture?
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Citrate test
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Indole test
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Methyl red test
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Rapid urease test
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Voges-Proskauer test
E
Correct answer
Explanation
Voges–Proskauer test is used to detect acetoin in bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red colour indicates a positive result, while a yellow-brown colour indicates a negative result.