Catalase Test: The catalase test is done by placing a drop of hydrogen peroxide on a microscope slide. If the bacteria possess catalase, when a small amount of bacterial isolate is added to hydrogen peroxide, bubbles of oxygen are observed. If the mixture produces bubbles or froth, the organism is said to be 'catalase-positive' otherwise it is 'catalase-negative’. Enterobacter and Klebsiella: Catalase-positive bacteria.