Multiple choice

Ion exchange chromatography is applicable for the separation of molecules on the basis of their charge. It is based on the reversible exchange ions in the solution with the ions bound to supportive media.

If you want to separate a positively charged protein using ion exchange chromatography, what kind of chromatography will be suitable?

  1. Anion exchange chromatography

  2. Cation exchange chromatography

  3. Both 1 and 2

  4. None of these
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

To separate a positively charged protein, we should use a negatively charged resin. For example, commonly we use the resin Phosphocellulose, which is a negatively charged resin for the cation exchange chromatography.

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