Multiple choice

A sample contains a mixture of proteins having approximately similar molecular masses. A particular protein of the sample tagged with histidine can be separated by which of the following techniques?

  1. Gel filtration chromatography

  2. Nickel affinity chromatography

  3. Ion exchange chromatography

  4. Affinity chromatography using Glutathion S-transferase

  5. All of the above

Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Affinity chromatography using Nickel can be used to separate protein tagged by histidine.