Multiple choice

Two proteins are having the same molecular mass and identical pH of 7. The best way to separate them will be

  1. SDS polyacrylamide gel electrophoresis

  2. Native gel electrophoresis

  3. Cation exchange chromatography

  4. Anion exchange chromatography
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

SDS PAGE is not used in if a particular protein has to be separated in the basis of their biological activity. As there is no treatment with SDS, all the proteins will carry their own charge at the pH and will move according to their different electrophoretic mobilities.

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