Multiple choice The chromatography technique used for the separation of stereoisomers is ion exchange chromatography size-exclusion chromatography reversed-phase chromatography chiral chromatography Reveal answer Fill a bubble to check yourself D Correct answer Explanation Chiral chromatography involves the separation of stereoisomers.To enable chiral separation to take place, either the mobile phase or the stationary phase must be made chiral, giving differing affinities between the analytes.