Two microbes found to be very useful in genetic engineering are-
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Escherichia coli and Agrobacterium tumefaciens
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Vibrio cholerae and a tailed bacteriophage
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Diplococcus sp.and Pseudomonas sp.
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Crown gall bacterium and Caenorhabditis
elegans
Reveal answer
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A
Correct answer
Explanation
- E. coli is a perfect model for several reasons: E. coli is a single-celled organism that can be manipulated and killed with no ethical concerns. It has a rapid growth rate and is very easy to culture and grow. Finally, and most importantly, E. coli genetics are well-studied and can be manipulated easily.
- The basis of Agrobacterium-mediated genetic engineering is that the T-DNA of A. tumefaciens is excised and integrates into the plant genome as part of the natural infection process by this bacterium. An Agrobacterium cell to act as the vehicle for a transforming plasmid. a Ti plasmid with functional Vir genes.
So, the correct answer is 'Escherichia coli and Agrobacterium tumefaciens'.