Tag: common modes of vegetative propagation

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Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Callus is _____________.

  1. Tissue that forms embryo

  2. An insoluble carbohydrate

  3. Tissue that grows to from embryoid

  4. Unorganised actively dividing mass of cells maintained in culture
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Callus is defined in plant tissue culture as an unorganized, proliferating mass of parenchyma cells that arises from an explant. It is typically maintained on a culture medium containing specific hormones.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

A totipotent cell means ________________.

  1. An undifferentiated cell capable of developing into a system or entire plant

  2. An undifferentiated cell capable of developing into an organ

  3. An undifferentiated cell capable of developing into complete embryo

  4. Cell which lacks the capability to differentiate into an organ or system.
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation
A totipotent cell means an undifferentiated cell capable of developing into a system or entire plant.
Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

The structure employed by White for first successful tissue culture was ____________.

  1. Root of Carrot

  2. Root of Tomato

  3. Leaf cells

  4. Apical meristem.
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

White was the first one to successfully attempt the technique of tissue culture. He cultured tomato roots in a nutrient media composed of vitamins. He developed the continuously growing callus from the root which had the property of totipotency.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Plant medium used widely in preparation of culture medium is got from ____________.

  1. Cycas revoluta

  2. Cocos nucifera

  3. Pinus roxburghii

  4. Borassus flabellifera
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Option $B$ is correct.

Plant medium used widely in the preparation of culture medium is got from Cocos nucifera milk of coconut or coconut water is widely used for culture preparation as it contains several growth substances and can be extracted easily. It provides the growth hormone known as cytokinin. Cytokinins promote cell division and induce morphogenesis. The first natural cytokinin known as zeatin was obtained from the coconut plant.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Banana can be rapidly multiplied by ____________.

  1. Multiple shoot production

  2. Embryoid formation

  3. Shoot tip culture

  4. Callus formation
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Banana is commercially and rapidly multiplied in vitro primarily through multiple shoot production, often initiated from shoot tip cultures. This method allows for the production of many genetically identical plantlets.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Haploids are useful for ________________.

  1. Production of callus

  2. Production of homozygous diploids

  3. Microdensitometer studies

  4. Induction of giant cells
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Haploid plant cultures are primarily used for generating homozygous doubled haploids through chromosome doubling, which rapidly produces pure lines for breeding. Callus is formed from explants rather than being a specific use of haploids, and the other options do not relate to haploid utility.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Which of the following statements are correct?
a. Callus is the disorganised mass of cells formed under in vitro condition.
b. Totipotency is the ability of a plant organ to regenerate an entire organism when cultured in a suitable medium.
c. Embryoid is the embryo-like structure formed under in vitro condition which has the potentiality to develop into a plantlet.
d. Explant is the excised portion of plant tissue or organ which is used in tissue culture study.
Select the correct answer using the codes given Codes.

  1. $1, 2$ and $4$
  2. $1, 3$ and $4$
  3. $1, 2$ and $3$
  4. $2, 3$ and $4$
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Statements 1, 3, and 4 correctly define callus as a disorganised mass of cells, embryoid as an embryo-like structure formed in vitro, and explant as the excised plant tissue. Statement 2 is slightly imprecise or often excluded in specific code selections because totipotency refers to the genetic potential of a cell rather than just an organ.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

The callus is defined as ________________.

  1. Decaying cells

  2. Differentiated cells

  3. Sterile mass of cells

  4. Undifferentiated mass of cells
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

A callus is an unorganized and undifferentiated mass of proliferating plant cells formed when an explant is cultured in vitro. Differentiated cells form specific tissues, while decaying cells refer to dead matter.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Electroporation is a technique commonly used in ________________.

  1. Tissue culture

  2. Somatic hybridization

  3. Radioimmuno assay

  4. Electrophoresis
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Electroporation is a technique used to introduce foreign DNA into cells by applying a high-voltage electrical pulse to create temporary pores in the cell membrane. It is a standard method in plant and animal tissue culture for transformation.