Tag: dna fingerprinting

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Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

In DNA finger printing, analysis is made of

  1. Satellite DNA

  2. Moderately repetitive sequences

  3. Microsatellites

  4. Variable number of tandem repeats
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

DNA fingerprinting relies on detecting variations in specific repetitive DNA regions. Variable Number of Tandem Repeats (VNTRs) are highly polymorphic sequences used as markers for individual identification.

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

Process used for amplication or multiplication of DNA for finger printing is

  1. Polymerase chain reaction

  2. Nesslerisation

  3. Southern blotting

  4. Northern blotting
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Polymerase Chain Reaction (PCR) is the standard laboratory technique used to amplify small segments of DNA, making them sufficient for analysis in fingerprinting.

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

The probes for DNA finger printing are

  1. Unknown single stranded labelled DNA

  2. Unknown double stranded labelled DNA

  3. Known single stranded labelled DNA

  4. Known double stranded unlabelled DNA
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Probes used in DNA fingerprinting are known, single-stranded DNA sequences that are radioactively or fluorescently labeled to bind specifically to complementary target sequences on the membrane.

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

Select the correct sequences of steps in DNA finger printing involving Southern blot hybridisation using radiolabelled VNTR as proble
I. Hybridisation using labelled VNTR prob
II. Isolation of DNA
III. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon
IV. Detection of hybridised DNA fragments by autoradiography
V. Separation of DNA fragments by electropheresis
VI. Digestion of DNA by restriction endonucleases

  1. I, V, VI, II, III and IV

  2. II, VI, V, III, I and IV

  3. V, I, VI, III, IV and II

  4. II, I, V, VI, IV and III
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The correct sequence is: Isolation of DNA (II), Digestion by restriction enzymes (VI), Separation by electrophoresis (V), Transfer/blotting (III), Hybridization (I), and Detection by autoradiography (IV).

Multiple choice zoology mutation dna fingerprinting human genome project and dna fingerprinting instruments in biotechnology

Repeated genes are useful

  1. Against mutations

  2. Formation of pseudogenes

  3. Formation of multiple copies of a product

  4. All of the above
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Repeated DNA sequences can act as more or less discrete elements in the genome. They can vary in the number of copies of repeat units from one chromosome to the next (polymorphism). The repeat copy number can expand through a unique form of mutation process referred to as ‘dynamic mutation.’ They are useful in the formation of pseudogenes and also in making multiple copies of a product.

So, the correct answer is 'All of the above.'