Tag: the origin of cells and cell division

Questions Related to the origin of cells and cell division

Which one of the following hydrolyze internal phosphodiester bonds in a polynucleotide chain?

  1. Lipase

  2. Protease

  3. Exonuclease

  4. Endonuclease


Correct Option: D
Explanation:

Endonucleases hydrolyse internal phosphodiester bonds in a polynucleotide chain. Function of nucleases is to break phosphodiester bond. A nuclease is an enzyme capable of cleaving the phosphodiester bonds between the nucleotide subunits of nucleic acids. Nucleases are usually further divided into endonucleases and exonucleases, although some of the enzymes may fall in both categories. 

Who coined the term enzyme?

  1. Pasteur

  2. Buchner

  3. Kuhne

  4. Sumner


Correct Option: C
Explanation:

Enzymes are biological catalysts, which activate various biochemical reactions of a living cell in a highly specific and precise manner. Enzymology is the study of enzymes. The name enzyme was coined by Kuhne in 1878. Pasteur recognised that some microorganisms like yeasts have got a capacity to cause fermentation in wine. In 1897, Buchner discovered that yeast extract could bring about fermentation of grape juice, like the living yeast cells. He also observed that the extract has lost its catalytic activity on boiling. He coined the word zymase for the active principle involved in the fermentation. The substance on which the enzyme acts is called substrate. Enzymes are essentially proteins but all proteins are not enzymes.

What did Kuhne do?

  1. Discovered parathyroid

  2. Coined the term enzyme

  3. Coined the term gene

  4. Discovered enzyme


Correct Option: B
Explanation:
  • In 1897, Eduard Buchner discovered that yeast extracts could ferment sugar to alcohol, proving that fermentation was promoted by molecules that continued to function when removed from cells. Frederick W. Khne called these molecules like enzymes.
    Hence, the correct option is B.

Enzymes that catalyse endergonic synthesis, coupled with exergonic hydrolysis of ATP are

  1. Ligases

  2. Isomerases

  3. Lyases

  4. Transferases


Correct Option: A
Explanation:
There are six major classes of enzymes, as follows:
(1) Oxidoreductases - Catalyze oxidationreduction reactions.
(2) Transferases - Catalyze the transfer of a functional group from a donor molecule to an acceptor molecule.
(3) Hydrolases - Catalyze hydrolysis reactions. 
(4) Isomerases - Catalyze conversion of a molecule from one isomeric form to another.
(5) Ligases - Ligases or synthetases are enzymes that catalyse endergonic synthesis, coupled with the exergonic hydrolysis of ATP
(6) Lyases - Catalyze certain reactions in which double bonds form or break.

Which enzyme act as chromosomal breaks?

  1. DNA polymerase

  2. DNase

  3. DNA ligase

  4. DNA polymerase II


Correct Option: B
Explanation:

The enzyme that cause chromosomal breaks are DNase. A deoxyribonuclease or DNase, is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA. Deoxyribonucleases are one type of nuclease, a generic term for enzymes capable of hydrolising phosphodiester bonds that link nucleotides.

Enzyme were discovered for the first time in

  1. Bacteria

  2. Yeast

  3. Algae

  4. Spinach


Correct Option: B
Explanation:

Enzymes are biological catalysts, which activate various biochemical reactions of a living cell in a highly specific and precise manner. Enzymology is the study of enzymes. The name enzyme was coined by Kuhne in 1878. Pasteur recognised that some microorganisms like yeasts have got a capacity to cause fermentation in wine. In 1897, Buchner discovered that yeast extract could bring about fermentation of grape juice, like the living yeast cells. He also observed that the extract has lost its catalytic activity on boiling. He coined the word zymase for the active principle involved in the fermentation. The substance on which the enzyme acts is called substrate. Enzymes are essentially proteins but all proteins are not enzymes.

Who coined the term zymase? 

  1. Pasteur

  2. Buchner

  3. Kuhne

  4. Sumner


Correct Option: B
Explanation:

Buchner coined the term zymase for the complex of biocatalysts extracted from yeast and taking part in alcoholic fermentation. Pasteur is responsible for the process of pasteurization and Kuhne is best known today for coining the word enzyme.

Which of the following set of three items are true, as each set belongs to the category mentioned against them?

  1. Lysine, glycine, thiamine - Amino acids

  2. Myosin, oxytocin and gastrin - Hormones

  3. Rennin, helicase and hyaluronidase - Enzyme

  4. Optic nerve, oculomotor, vagus - Sensory nerves


Correct Option: C
Explanation:

Rennin is a protease found in rennet. It is produced by newborn ruminant animals in the lining of the fourth stomach to curdle the milk they ingest, allowing a longer residence in the bowels and better absorption. It is widely used in the production of cheese.

Helicases are enzymes that bind and may even remodel nucleic acid or nucleic acid protein complexes. There are DNA and RNA helicases. DNA helicases are essential during DNA replication because they separate double-stranded DNA into single strands allowing each strand to be copied.
Hyaluronidase is a protein enzyme. It works by causing rapid spreading of injected fluid into the body, which increases the absorption of injected fluids.
Therefore, the correct answer is option C.

Enzyme concerned with transfer of electrons are

  1. Hydrolase

  2. Dehydrogenase

  3. Transaminase

  4. Protease


Correct Option: B
Explanation:

Dehydrogenases are the enzymes which catalyze oxidation-reduction reactions by addition or removal of electrons. These reactions are used to synthesize reduced coenzymes like NADH$ _2$ and FADH$ _2$. For example phosphoglyceraldehyde dehydrogenase.

So, the correct answer is 'Dehydrogenase'.

Which enzyme shows greatest substrate specificity?

  1. Nuclease

  2. Trypsin

  3. Sucrase

  4. Pepsin


Correct Option: C
Explanation:
Enzymes are highly specific catalysts and show high degree of substrate specificity. Some enzymes names are general and these enzymes act on a type of biomolecules. For example: nucleases means enzymes acting on nucleic acids. Nuclease is a general class of enzyme. Similarly, pepsin and trypsin are protein metabolising enzymes. These enzymes will act on any proteins and not on some specific protein. Sucrase is, however, a specific enzyme which will act on sucrose only.
So, the correct answer 'Sucrase'