Tag: biotechnology and its applications

Quizzes (13) Questions (226)

Questions Related to biotechnology and its applications

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

Which of the following is not used to transfer the recombinant DNA into the host?

  1. Micro-injection method

  2. Gene gun method

  3. Bioreactors

  4. Disarmed pathogen vectors
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation
Bioreactors are considered as vessels in which raw materials are biologically converted into specific products by microbes, plant and animal cells or their enzymes. To produce large quantities of these products, bioreactors are used where large volumes (100-1000 litres) of culture can be processed. Bioreactor provides the optimal conditions for obtaining the desired product by providing optimum growth conditions such as temperature, pH, substrate, vitamins, oxygen and salts.
So, the correct answer is 'Bioreactors'.
Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

Read the following four statements (A-D).

A. The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched.
B. Restriction enzymes are used in isolation of DNA from other macromolecules.
C. Downstream processing is one of the steps of rDNA technology.
D. Disarmed pathogen vectors are also used in transfer of rDNA into the host.

Which of the two statements have mistakes?

  1. B and C

  2. C and D

  3. A and C

  4. A and B
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Statement A is incorrect because Rosie was a transgenic cow, not a buffalo. Statement B is incorrect because restriction enzymes are used to cut DNA at specific sites, not for the isolation of DNA from other macromolecules (which is done via chemical extraction).

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

Which of the following is not a benefit of transgenic animals?

  1. Investigation of new treatments for diseases

  2. Early detection of diseases

  3. Testing the safety of vaccines

  4. To produce useful biological products
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Early diagnosis of disease cannot be done by transgenic animals but through techniques such as PCR and ELISA.

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

Inbreeding for five generations led to production of homozygous transgenic mice. However, these homozygous males or females were infertile. Which of the following approaches is most preferable and economical to obtain heterozygous transgenic animals continuously?

  1. More transgenic founder (1st animal) should be generated.

  2. Crossing (breeding) of transgenic mice with wild type mice in earlier generations should be done for continued production of transgenic heterozygous offsprings

  3. Inbreeding should be avoided after 5th generation

  4. Homozygous transgenic mice should be mated with heterozygous transgenic mice for continued production of transgenic heterozygous offsprings.
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Interbreeding of particular species for a larger amount of time causes the recessive traits to appear in the offspring and those result in infertility. Inbreeding is not recommended and cross-breeding is required. When the transgenic mice are inbred for very large duration infertility is caused.

To get heterozygous transgenic mice crossbreeding should be done with the wild-type.
So, the correct option is 'Crossing (breeding) of transgenic mice with wild type mice in earlier generations should be done for continued production of transgenic heterozygous offsprings'

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

Select the incorrect matched pair.

  1. Monoclonal antibodies - Hybridomas

  2. PCR - Phenylketonuria

  3. Bioweapons - Bacillus anthracis

  4. Tracy - First transgenic animal for food production
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Tracy is a transgenic sheep, but the term 'first transgenic animal for food production' is not the standard descriptor for her; she was designed for pharmaceutical protein production (molecular farming). However, in the context of typical exam distractors, this is the intended incorrect pair compared to the others which are standard textbook associations.

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

A human protein which is obtained from transgenic animals and is used to treat emphysema is 

  1. Alpha-lactalbumin

  2. Thyroxine
  3. $\alpha-1$-antitrypsin

  4. Insulin.
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

A human protein which is obtained from transgenic animals and is used to treat emphysema is α−1-antitrypsin. Transgenic animals that produce useful biological products can be created by the introduction of the portion of DNA which codes for particular product such as human proteins. 

So, the correct option is 'Option C' .

Multiple choice zoology biotechnology and its applications transgenic animals transgenic organisms applications of biotechnology

Given below are certain features of mice. Read them and select why mice is most preferred animal for studies on gene transfer.


(i) Short estrous cycle and gestation period
(ii) Relatively short generation time
(iii) Convenient in vitro fertilisation
(iv) Production of several offspring per pregnancy 

  1. (i) and (ii) only

  2. (i) only

  3. (i), (ii) and (iv) only

  4. (i), (ii), (iii) and (iv)
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Mouse is the most preferred animal for studies on gene transfer due to many favourable features like short oestrous cycle and gestation period, relatively short generation time, production of several offspring per pregnancy, convenient in vitro fertilisation, successful culture of embryo in vitro, etc.