Tag: genetic technology

Quizzes (15) Questions (44)

Questions Related to genetic technology

Multiple choice botany genetic technology dna transfer rdna technology - dna transfer and transformation gene cloning procedure

The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of

  1. Inactivation of glycosidase enzyme in recombinant bacteria

  2. Nonrecombinant bacteria contain beta galactosidase
  3. Insertional inactivation of $\alpha$-galactosidase in nonrecombinant bacteria
  4. Insertional inactivation of $\alpha$-galactosidase in recombinant bacteria
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

In blue-white screening, the insertion of foreign DNA into the lacZ gene (which encodes beta-galactosidase) causes insertional inactivation, preventing the production of the enzyme and resulting in white colonies.

Multiple choice botany genetic technology dna transfer rdna technology - dna transfer and transformation gene cloning procedure biotechnology in agriculture

Changes in transcription after linking the cloned DNA of the putative promoter/enhancer region to cloned DNA of the coding region of an unrelated gene, can be measured rapidly with a high degree of sensitivity using.

  1. Transgenic technology

  2. Reporter gene technology

  3. cDNA technology

  4. Gene transfer technology
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Reporter gene technology links a putative promoter or enhancer region to the coding region of an easily assayable reporter gene, allowing rapid and sensitive measurement of changes in transcription levels.

Multiple choice botany genetic technology dna transfer rdna technology - dna transfer and transformation gene cloning procedure

Which one of the following techniques made it possible to genetically engineer living organisms?

  1. Heavier isotope labelling

  2. Hybridization

  3. Recombinant DNA techniques

  4. X-ray diffraction
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a genetically modified organism (GMO) with a new genotype. Various kinds of genetic modification are possible: inserting a foreign gene from one species into another, forming a transgenic organism; altering an existing gene so that its product is changed; or changing gene expression so that it is translated more often or not at all.

Multiple choice biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

The centre of origin of wheat is

  1. Southeast Asia

  2. Southwest Asia

  3. Asia minor and Afganistan

  4. None of the above
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Wheat is grown on more land area worldwide than any other crop and is a close third to rice and corn in total world production. Wheat is well adapted to harsh environments and is mostly grown on wind swept areas that are too dry and too cold for the more tropically inclined rice and corn, which do best at intermediate temperature levels. 

Wheat is believed to have originated in southwestern Asia. Some of the earliest remains of the crop have been found in Syria, Jordan and Turkey. Primitive relatives of present day wheat have been discovered in some of the oldest excavations of the world in eastern Iraq, which date back 9,000 years. Thus, the correct answer is option B.

Multiple choice biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

Nitrocellulose and nylon are used in which of the following technique of immobilisation?

  1. Entrapment

  2. Encapsulation

  3. Adsorption

  4. Cross-linking
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Encapsulation involves enclosing enzymes within a semi-permeable membrane, such as nitrocellulose or nylon, to immobilize them while allowing substrate and product diffusion.

Multiple choice biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

The pore size of the matrix used for immobilisation of enzymes depends upon

  1. Concentration of enzyme

  2. Molecular weight of enzyme

  3. Catalytic nature of enzyme

  4. Both A and B
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

The pore size of an immobilization matrix must be chosen to accommodate the enzyme's size (molecular weight) and ensure that the enzyme concentration is optimized for the reaction.

Multiple choice biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

Which of the following is a common use of somatic hybridization?

  1. Transfer of jumping genes

  2. Transfer of some of the chromosomes between divergent species

  3. Transfer of resistant gene

  4. None of the above
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The process by which protoplasts of two different plant species are fused together and transfer of some of the chromosomes occurs between two species to form hybrids is called as somatic hybridization and the hybrids that are produced is known as somatic hybrids. The steps of somatic hybridization involve - isolation of protoplasts, the fusion of different protoplasts and selection of hybrids. 

Thus, the correct answer is option B.