Tag: genetic technology

Questions Related to genetic technology

The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of

botany genetic technology dna transfer rdna technology - dna transfer and transformation gene cloning procedure

  1. Inactivation of glycosidase enzyme in recombinant bacteria

  2. Nonrecombinant bacteria contain beta galactosidase

  3. Insertional inactivation of $\alpha$-galactosidase in nonrecombinant bacteria

  4. Insertional inactivation of $\alpha$-galactosidase in recombinant bacteria

Show answer
Correct Option: D

Changes in transcription after linking the cloned DNA of the putative promoter/enhancer region to cloned DNA of the coding region of an unrelated gene, can be measured rapidly with a high degree of sensitivity using.

botany genetic technology dna transfer rdna technology - dna transfer and transformation gene cloning procedure biotechnology in agriculture

  1. Transgenic technology

  2. Reporter gene technology

  3. cDNA technology

  4. Gene transfer technology

Show answer
Correct Option: A

Which one of the following techniques made it possible to genetically engineer living organisms?

botany genetic technology dna transfer rdna technology - dna transfer and transformation gene cloning procedure

  1. Heavier isotope labelling

  2. Hybridization

  3. Recombinant DNA techniques

  4. X-ray diffraction

Show answer
Correct Option: C
Explanation:

Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a genetically modified organism (GMO) with a new genotype. Various kinds of genetic modification are possible: inserting a foreign gene from one species into another, forming a transgenic organism; altering an existing gene so that its product is changed; or changing gene expression so that it is translated more often or not at all.

The centre of origin of wheat is

biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

  1. Southeast Asia

  2. Southwest Asia

  3. Asia minor and Afganistan

  4. None of the above

Show answer
Correct Option: B
Explanation:

Wheat is grown on more land area worldwide than any other crop and is a close third to rice and corn in total world production. Wheat is well adapted to harsh environments and is mostly grown on wind swept areas that are too dry and too cold for the more tropically inclined rice and corn, which do best at intermediate temperature levels. 

Wheat is believed to have originated in southwestern Asia. Some of the earliest remains of the crop have been found in Syria, Jordan and Turkey. Primitive relatives of present day wheat have been discovered in some of the oldest excavations of the world in eastern Iraq, which date back 9,000 years. Thus, the correct answer is option B.

Nitrocellulose and nylon are used in which of the following technique of immobilisation?

biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

  1. Entrapment

  2. Encapsulation

  3. Adsorption

  4. Cross-linking

Show answer
Correct Option: B

The carriers or supporters which are used for covalent bonding for immobilisation of enzymes?

biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

  1. Cellulose

  2. Collagen

  3. Gelatin

  4. All of the above

Show answer
Correct Option: D

The pore size of the matrix used for immobilisation of enzymes depends upon

biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

  1. Concentration of enzyme

  2. Molecular weight of enzyme

  3. Catalytic nature of enzyme

  4. Both A and B

Show answer
Correct Option: D

Which of the following is a method of adsorption used for immobilisation of enzymes?

biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

  1. Reactor loading process

  2. Electrode position process

  3. Diazoation

  4. Both A and B

Show answer
Correct Option: D

Which of the following can be used as matrixes for entrapment?

biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

  1. Agar

  2. Gelatin

  3. Cellulose triacetate

  4. All of the above

Show answer
Correct Option: D

Which of the following is a common use of somatic hybridization?

biology biotechnology and genetic engineering making use of enzymes genetic technology genetic engineering

  1. Transfer of jumping genes

  2. Transfer of some of the chromosomes between divergent species

  3. Transfer of resistant gene

  4. None of the above

Show answer
Correct Option: B
Explanation:

The process by which protoplasts of two different plant species are fused together and transfer of some of the chromosomes occurs between two species to form hybrids is called as somatic hybridization and the hybrids that are produced is known as somatic hybrids. The steps of somatic hybridization involve - isolation of protoplasts, the fusion of different protoplasts and selection of hybrids. 

Thus, the correct answer is option B.