Tag: bio-chemistry

Questions Related to bio-chemistry

What is true about DNA polymerase used in PCR?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. It is used to ligate introduced DNA in recipient cells.

  2. It serves as selectable marker.

  3. It is isolated from a virus.

  4. It is active at high temperature.

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Correct Option: D

Melting of DNA at 70$^o$ C is due to breakdown of

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Phosphodiester bonds

  2. Hydrogen bonds

  3. Glycosidic bonds

  4. Disulphide bonds

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Correct Option: B

Amplification of gene of interest by using PCR may go upto

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. 0.1 million

  2. 1.0 million

  3. 1.0 billion

  4. 1.0 trillion

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Correct Option: C

At which temperature a DNA molecule is denatured by heat in PCR

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. 70$^o$ -  80$^o$C

  2. 90$^o$ - 95$^o$C

  3. 42$^o$C

  4. 50$^o$ - 65$^o$C

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Correct Option: B

Taq polymerase is isolated from a

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Virus

  2. Bacterium

  3. Fungus

  4. Protozoan

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Correct Option: B

Taq polymerase is isolated from which bacteria?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. E. coli

  2. Bacillus thuringiensis

  3. Thermus aquaticus

  4. Agrobacterium

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Correct Option: C

Polymerase chain reaction (PCR) is used for

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. In vivo replication of specific DNA sequence using thermostable DNA polymerase

  2. In vitro synthesis of mRNAIn vivo synthesis of mRNA

  3. In vitro replication of specific DNA sequence using thermostable DNA polymerase

  4. Separation of DNA fragments according to their size

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Correct Option: C

Which one of the following is NOT a PCR-based molecular marker?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. RAPD

  2. AP-PCR

  3. DAF

  4. RELP

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Correct Option: A

DNA is amplified by __________________.

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. PCR technique

  2. Southern Blotting technique

  3. Northern Blotting technique

  4. Nucleic acid hybridization technique

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Correct Option: A

Which one of the following enzymes is used in PCR reaction?

bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

  1. Taq polymerase

  2. RNA polymerase

  3. Rib nuclease

  4. End nuclease

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Correct Option: A