Tag: bio-chemistry

Quizzes (82) Questions (1918)

Questions Related to bio-chemistry

Multiple choice bio-chemistry digestive system of human absorption of food absorption parts of the digestive system: structure and function

Choose the correct answer from the allternatives given.
What is the name of the portion of alimentary canal which crushes the food ?

  1. Crop

  2. Gizzard

  3. Oesophagus

  4. Stomach
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Answer B: Gizzard

Stomach constructed of thick muscular walls is used for grinding up food, often aided by particles of stone or grit. In certain insects, the gizzard features chitinous plates or teeth.

Multiple choice bio-chemistry digestion absorption of food absorption parts of the digestive system: structure and function

Secretin is gastrointestinal tract hormone that

  1. Acts on exocrine part of pancreas and stimulates the secretion of water and bicarbonate ions

  2. Acts on exocrine portion of pancreas and stimulates the cells to secrete glucagon

  3. Acts on gastric glands and stimulates secretion of HCl and Pepsinogen

  4. Stimulates secretion of gastric lipase from stomach
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Secretin is released by the duodenum in response to acidic chyme. It travels through the blood to the pancreas, where it stimulates the secretion of water and bicarbonate ions to neutralize the acid.

Multiple choice bio-chemistry digestive system of human absorption of food absorption parts of the digestive system: structure and function

Which of the following is the primary absorptive process in the large intestine?

  1. Active transport of $Na^+$ from the lumen to the blood

  2. Absorption of amino acids and fructose

  3. Active transport of potassium from the lumen to the blood
  4. Active absorption of $HCO _3$ into the blood
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The primary function of the large intestine regarding absorption is the reabsorption of water and electrolytes, specifically the active transport of sodium ions from the lumen into the blood, which creates an osmotic gradient for water absorption.

Multiple choice bio-chemistry digestion absorption of food absorption parts of the digestive system: structure and function

Choose the odd one w.r.t. locations of source cells for hormone.

  1. Gastrin

  2. Enterogastrone

  3. Secretin

  4. Duocrinin
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The gastrin hormone is secreted by the pyloric stomach located adjacent to the duodenum. This hormone stimulates the gastric glands to release gastric juice and regulates the movement of the stomach. 

Secretin is produced by the small intestinal mucosa region. It may inhibit the gastrin secretion and stomach movement. Both enterogastrone and duocrinin hormones are released from duodenal mucosa. Enterogastrone regulates gastric contraction and inhibits production of gastric juice. Duocrinin stimulates the viscous mucosa secretion from Brunner's gland into the intestinal juice. 
So, the correct answer is option A, Gastrin. 

Multiple choice bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would you find the smallest segments of DNA?

  1. Near the positive electrode, farthest away from the wells

  2. Near the negative electrode, close to the wells

  3. Near the negative electrode, farthest away from the wells

  4. Near the middle, they tend to slow down after the first few minutes
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Since DNA is itself negatively charged, it would move towards the positive electrode. In gel electrophoresis, DNA fragments are separated on the basis of charge and masses. Thus, smaller the DNA fragment farther it moves from the well.

So, the correct option is A.

Multiple choice bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

Gel electrophoresis is used for

  1. Construction of recombinant DNA by joining with cloning vectors

  2. Isolation of DNA molecules

  3. Cutting of DNA into fragments

  4. Separation of DNA fragments according to their size
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are separated according to their size. 

So, the correct option is 'Option D' .

Multiple choice bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

Select the correct option to fill up the blanks.


(i) _____ is a natural polymer extracted from ______.

(ii) The DNA fragments purified by gel electrophoresis are used in constructing _____ by joining them with ______.
(iii) The ligation of alien DNA is carried out at a _____ present in one of the two ____ in a plasmid vector.
(iv) _____ enzyme remains active during the high temperature-induced denaturation of ds DNA. DNA fragments are resolved according to their ______
(v) through _____ in agarose gel electrophoresis.

  1. (i) Agarose, seaweeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase, size(v) sieving effect.

  2. (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect

  3. (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii A-DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect

  4. (i) Size, sieving effect (ii) agarose, sea weeds (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Agarose is a natural polymer extracted from sea weeds. The DNA fragments purified by gel electrophoresis are used in constructing recombinant DNA by joining them with the cloning vector. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes in a plasmid vector. Taq polymerase enzyme remains active during the high temperature-induced denaturation of dsDNA.  DNA fragments are resolved according to their size through a sieving effect in agarose gel electrophoresis.

So, the correct option is 'Option A'.