Tag: bio-chemistry

Questions Related to bio-chemistry

Germs entering the body along with food are killed in alimentary canal where pH is

bio-chemistry digestive system of human absorption of food absorption parts of the digestive system: structure and function

  1. 10

  2. 7

  3. 3

  4. 11

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Correct Option: C

Choose the correct answer from the allternatives given.
What is the name of the portion of alimentary canal which crushes the food ?

bio-chemistry digestive system of human absorption of food absorption parts of the digestive system: structure and function

  1. Crop

  2. Gizzard

  3. Oesophagus

  4. Stomach

Show answer
Correct Option: B
Explanation:

Answer B: Gizzard

Stomach constructed of thick muscular walls is used for grinding up food, often aided by particles of stone or grit. In certain insects, the gizzard features chitinous plates or teeth.

Secretin is gastrointestinal tract hormone that

bio-chemistry digestion absorption of food absorption parts of the digestive system: structure and function

  1. Acts on exocrine part of pancreas and stimulates the secretion of water and bicarbonate ions

  2. Acts on exocrine portion of pancreas and stimulates the cells to secrete glucagon

  3. Acts on gastric glands and stimulates secretion of HCl and Pepsinogen

  4. Stimulates secretion of gastric lipase from stomach

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Correct Option: A

Which of the following is the primary absorptive process in the large intestine?

bio-chemistry digestive system of human absorption of food absorption parts of the digestive system: structure and function

  1. Active transport of $Na^+$ from the lumen to the blood

  2. Absorption of amino acids and fructose

  3. Active transport of potassium from the lumen to the blood

  4. Active absorption of $HCO _3$ into the blood

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Correct Option: A

Choose the odd one w.r.t. locations of source cells for hormone.

bio-chemistry digestion absorption of food absorption parts of the digestive system: structure and function

  1. Gastrin

  2. Enterogastrone

  3. Secretin

  4. Duocrinin

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Correct Option: A
Explanation:

The gastrin hormone is secreted by the pyloric stomach located adjacent to the duodenum. This hormone stimulates the gastric glands to release gastric juice and regulates the movement of the stomach. 

Secretin is produced by the small intestinal mucosa region. It may inhibit the gastrin secretion and stomach movement. Both enterogastrone and duocrinin hormones are released from duodenal mucosa. Enterogastrone regulates gastric contraction and inhibits production of gastric juice. Duocrinin stimulates the viscous mucosa secretion from Brunner's gland into the intestinal juice. 
So, the correct answer is option A, Gastrin. 

The hormone responsible for vagus nerve activity is

bio-chemistry digestion absorption of food absorption parts of the digestive system: structure and function

  1. CCK

  2. Secretin

  3. Gastrin

  4. GIP

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Correct Option: C

Which one of the following does not match is biological category of chemical substance.

bio-chemistry digestion absorption of food absorption parts of the digestive system: structure and function

  1. Gastrin

  2. Creatinine

  3. Oxytocin

  4. Renin

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Correct Option: A

Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would you find the smallest segments of DNA?

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Near the positive electrode, farthest away from the wells

  2. Near the negative electrode, close to the wells

  3. Near the negative electrode, farthest away from the wells

  4. Near the middle, they tend to slow down after the first few minutes

Show answer
Correct Option: A
Explanation:

Since DNA is itself negatively charged, it would move towards the positive electrode. In gel electrophoresis, DNA fragments are separated on the basis of charge and masses. Thus, smaller the DNA fragment farther it moves from the well.

So, the correct option is A.

Gel electrophoresis is used for

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. Construction of recombinant DNA by joining with cloning vectors

  2. Isolation of DNA molecules

  3. Cutting of DNA into fragments

  4. Separation of DNA fragments according to their size

Show answer
Correct Option: D
Explanation:

Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins. DNA fragments are separated according to their size. 

So, the correct option is 'Option D' .

Select the correct option to fill up the blanks.


(i) _____ is a natural polymer extracted from ______.

(ii) The DNA fragments purified by gel electrophoresis are used in constructing _____ by joining them with ______.
(iii) The ligation of alien DNA is carried out at a _____ present in one of the two ____ in a plasmid vector.
(iv) _____ enzyme remains active during the high temperature-induced denaturation of ds DNA. DNA fragments are resolved according to their ______
(v) through _____ in agarose gel electrophoresis.

bio-chemistry biochemical techniques manipulation of dna electrophoresis isolation and separation of dna

  1. (i) Agarose, seaweeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase, size(v) sieving effect.

  2. (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect

  3. (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii A-DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect

  4. (i) Size, sieving effect (ii) agarose, sea weeds (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

Show answer
Correct Option: A
Explanation:

Agarose is a natural polymer extracted from sea weeds. The DNA fragments purified by gel electrophoresis are used in constructing recombinant DNA by joining them with the cloning vector. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes in a plasmid vector. Taq polymerase enzyme remains active during the high temperature-induced denaturation of dsDNA.  DNA fragments are resolved according to their size through a sieving effect in agarose gel electrophoresis.

So, the correct option is 'Option A'.