Biology
Genetic Engineering and Biotechnology
2,033 Questions
Genetic engineering and biotechnology involve modifying organism DNA to develop transgenic crops, medical treatments, and industrial solutions. This field covers essential techniques like hybridoma technology, molecular markers, and gene transfer in eukaryotic cells. These biology topics are essential for aspirants preparing for various competitive examinations.
Genetic engineering techniquesTransgenic organismsIndustrial biotechnologyGMOs and bioremediation
Genetic Engineering and Biotechnology Questions
B
Correct answer
Explanation
PCR reaction is exponential. After 10 cycles of amplification, one expects approximately 210 copies, or around 1000.
A
Correct answer
Explanation
The yeast artificial chromosome can accommodate several hundred thousand base pairs to upwards of a million.
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Agglutination : Cross linkage of antigens by antibodies
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Hybridoma : Revealing intra-specific variation and diversity between species
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Plant tissue culture technique : Selection of somaclones that are resistant to herbicides
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RNA interference : Biological process in which RNA molecules inhibit gene expression
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Photolithography : Transferring geometric shapes on a mask to the surface of a silicon wafer
B
Correct answer
Explanation
This option is correct because Hybridoma technology is a technology of forming hybrid cell lines by fusing an antibody-producing B cell with a myeloma (B cell cancer) cell that is selected for its ability to grow in tissue culture and for an absence of antibody chain synthesis and revealing intra-specific variation and diversity between species is a Random Amplified Polymorphic DNA (RAPD).
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Epigenesis
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Mutagenesis
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Metastasis
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Apoptosis
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Carcinogenesis
C
Correct answer
Explanation
Metastasis refers to the spread of a cancer to different parts of the body and the appearance of secondary tumours.
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1, 2, 3, 4
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4, 1, 3, 2
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3, 1, 2, 4
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4, 3, 2, 1
D
Correct answer
Explanation
This is the correct sequence.
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molecular glue
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molecular scissors
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molecular markers
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reverse transcriptase
B
Correct answer
Explanation
Restriction endonucleases are also known as molecular scissors as they function in cutting the DNA strand.
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organisms
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cells
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molecular analogs
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All of the above
D
Correct answer
Explanation
According to EFB, biotechnology is the integration of natural sciences, organisms, cell parts and molecular analogs for products and services.
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Vector method
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PCR technique
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Bolistic method
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Plasmid method
C
Correct answer
Explanation
Bolistic method uses a gene gun for genetic engineering.
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Genetically manipulated organism
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Genetically modified organism
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Genetically maneuvered organism
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Generating mutated organism
B
Correct answer
Explanation
GMO stands for genetically modified organism.
C
Correct answer
Explanation
Andi was the first genetically altered primate born in October 2000.
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Genome editing
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Gene targeting
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Gene trapping
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Gene splicing
B
Correct answer
Explanation
Gene targeting is a genetic technique that uses homologous recombination to change an endogenous gene.
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Hybrid plants
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Transgenic plants
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Elite plants
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Improved varieties
B
Correct answer
Explanation
Plants modified by genetic engineering are called transgenic plants.
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Kyoto Expression of Genes and Genomes
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Kyoto Encyclopedia of Genes and Genomes
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Kyoto Expression of Genotypes and Genomes
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Kyoto Encyclopedia of Genes and Genotypes
B
Correct answer
Explanation
KEGG stands for Kyoto Encyclopedia of Genes and Genomes. It is a comprehensive database of biological pathways, diseases, drugs, and chemical substances. The name explicitly references 'Encyclopedia' not 'Expression' or 'Genotypes'.
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A chemical
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A structure guided drug
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A prospective drug
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All of these
D
Correct answer
Explanation
In drug discovery, 'leads' are prospective drug compounds that show promising activity and have potential for further development. They can be chemicals, structure-guided compounds, or various other molecular entities.
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similarity studies
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structure prediction
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profile search
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motif search
A
Correct answer
Explanation
BioEdit is a biological sequence alignment editor designed to facilitate nucleic acid and protein sequence alignment analysis, editing, and similarity studies. It is not primarily a structure prediction or motif search tool.