Biology

Genetic Engineering and Biotechnology

2,033 Questions

Genetic engineering and biotechnology involve modifying organism DNA to develop transgenic crops, medical treatments, and industrial solutions. This field covers essential techniques like hybridoma technology, molecular markers, and gene transfer in eukaryotic cells. These biology topics are essential for aspirants preparing for various competitive examinations.

Genetic engineering techniquesTransgenic organismsIndustrial biotechnologyGMOs and bioremediation

Genetic Engineering and Biotechnology Questions

Multiple choice

It can be dangerous to opt for genetic engineer because ___________________.

Directions: Answer the question based on the following passage.

Now that we have decoded the human genome, why don't we improve it?
The question is at present theoretical but could well emerge as the hardest of all bioethical issues. Biologists routinely alter the genes of mice, with methods that are not yet acceptable for making inheritable changes in people, but one day genetic engineers may figure out how to apply safe patches to the human biological software.
Everyone would like to have children who are healthy, beautiful and gifted. But people vary widely in all these qualities, depending on their parents' genes, and the pure luck of the draw at conception when each child gets allotted a random selection of half the parental gene pool.
Most human genes exist in several different versions in the population: some of them are great to have, some so-so and some downright deleterious.
This month the Icelandic company Decode Genetics found three quite common versions of a gene called BMP-2, each of which considerably increases its owner's risk of osteoporosis and bone fracture.
Suppose it was possible to delete any bad version of BMP-2, and of all other human genes, in a human embryo, and to replace them with good versions, without any risk to health. Would that be the right thing to do?
Parents who made such a choice would know they had given their child the best possible start in life. However expensive the procedure, it would be cheap in the long run if it saved a lifetime of medical bills, and therefore could be made available to all. Life's most serious unfairness, the difference in genetic endowment, would be erased from birth.
"One day, people may view sex as essentially recreational, and conception as something best done in the laboratory," Dr. Gregory Stock wrote recently in "Redesigning Humans.” Parents may start to believe it is "reckless and primitive to conceive a child without prior genetic testing."
Yet there are weighty arguments for not making inheritable changes to the human genome.
On the practical side, many genes have more than one effect, and swapping out the bad version of a gene can have unpredictable complications. The new gene, for example, may interact badly with the person's other genes.
But if the elimination of disease-causing variants of genes should prove successful, there might be no holding the line against parents who wanted to enhance strength or intelligence as well.
Upgrading the imperfect human material is all very well, but handling the transition between the super people and the ordinary variety promises to be awkward. Social stresses may emerge, especially if the technology does not trickle down quickly and smoothly.
Soup up those genes for I.Q.? Altering the genes that shape human behavior is not to be 0undertaken lightly. Human nature is a subtle blend of contrary qualities, the only survivor of evolution's many disastrous experiments. What could justify the risk of messing with such a delicate brew? Can't we be happy as we are, just as nature has shaped us?
"The human body and mind, highly complex and delicately balanced as a result of eons of gradual and exacting evolution, are almost certainly at risk from any ill-considered attempt at `improvement,' " the President's Council on Bioethics wrote in a report last month on the dangers of enhancing the body's natural abilities.
As the products of evolution, people may seem churlish if they challenge evolution's wisdom. But of course, evolution has none. It is a blind process that depends on constant error to create occasional lucky accidents.
By culling the unfortunate owners of bad genes, evolution keeps animals healthy and vigorous until the age of reproduction, and a bit beyond for species that provide parental care.
But evolution's rigor at favoring good genes that act early in life is mirrored by a weakness in screening out bad genes that act after the age of reproduction. Because of this weakness, evolution has failed to eliminate the bone-fracturing variants of BMP-2, and the bad, late-acting versions of many other genes in the human genome. This is the very reason that we age and die.
If evolution cannot help us after a certain age, why should we not help ourselves? Should not everyone have a right to the best versions of the genes in our collective genetic heritage, or at least to be born free of the worse ones?
And yet, if we reduce genetic differences, we risk turning the human population into one giant clone, tedious to meet with and bereft of the variation needed to respond to changing environments. The pursuit of perfection, if carried to extremes, is sure recipe for extinction.

 

  1. it defies the ethical constructs

  2. it augments the detrimental effects of an arbitrary genetic combination

  3. it would reduce he procreational value of sex

  4. it would topple the delicate balance of evolution

  5. it could lead to various socio-cultural complications

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

(4) is the right answer as, (1) is one of the negative effects of Genetic Engineering but it can hardly be called dangerous. (2) is incorrect because Genetic Engineering decreases and not increases the negative effects of a natural combination. (3) again would not be dangerous. (5) only stress on the socio-cultural scenario while (4) covers the conditions.

Multiple choice

Genetic Engineering can have all the following functions except ____________________.

Directions: Answer the question based on the following passage.

Now that we have decoded the human genome, why don't we improve it?
The question is at present theoretical but could well emerge as the hardest of all bioethical issues. Biologists routinely alter the genes of mice, with methods that are not yet acceptable for making inheritable changes in people, but one day genetic engineers may figure out how to apply safe patches to the human biological software.
Everyone would like to have children who are healthy, beautiful and gifted. But people vary widely in all these qualities, depending on their parents' genes, and the pure luck of the draw at conception when each child gets allotted a random selection of half the parental gene pool.
Most human genes exist in several different versions in the population: some of them are great to have, some so-so and some downright deleterious.
This month the Icelandic company Decode Genetics found three quite common versions of a gene called BMP-2, each of which considerably increases its owner's risk of osteoporosis and bone fracture.
Suppose it was possible to delete any bad version of BMP-2, and of all other human genes, in a human embryo, and to replace them with good versions, without any risk to health. Would that be the right thing to do?
Parents who made such a choice would know they had given their child the best possible start in life. However expensive the procedure, it would be cheap in the long run if it saved a lifetime of medical bills, and therefore could be made available to all. Life's most serious unfairness, the difference in genetic endowment, would be erased from birth.
"One day, people may view sex as essentially recreational, and conception as something best done in the laboratory," Dr. Gregory Stock wrote recently in "Redesigning Humans.” Parents may start to believe it is "reckless and primitive to conceive a child without prior genetic testing."
Yet there are weighty arguments for not making inheritable changes to the human genome.
On the practical side, many genes have more than one effect, and swapping out the bad version of a gene can have unpredictable complications. The new gene, for example, may interact badly with the person's other genes.
But if the elimination of disease-causing variants of genes should prove successful, there might be no holding the line against parents who wanted to enhance strength or intelligence as well.
Upgrading the imperfect human material is all very well, but handling the transition between the super people and the ordinary variety promises to be awkward. Social stresses may emerge, especially if the technology does not trickle down quickly and smoothly.
Soup up those genes for I.Q.? Altering the genes that shape human behavior is not to be 0undertaken lightly. Human nature is a subtle blend of contrary qualities, the only survivor of evolution's many disastrous experiments. What could justify the risk of messing with such a delicate brew? Can't we be happy as we are, just as nature has shaped us?
"The human body and mind, highly complex and delicately balanced as a result of eons of gradual and exacting evolution, are almost certainly at risk from any ill-considered attempt at `improvement,' " the President's Council on Bioethics wrote in a report last month on the dangers of enhancing the body's natural abilities.
As the products of evolution, people may seem churlish if they challenge evolution's wisdom. But of course, evolution has none. It is a blind process that depends on constant error to create occasional lucky accidents.
By culling the unfortunate owners of bad genes, evolution keeps animals healthy and vigorous until the age of reproduction, and a bit beyond for species that provide parental care.
But evolution's rigor at favoring good genes that act early in life is mirrored by a weakness in screening out bad genes that act after the age of reproduction. Because of this weakness, evolution has failed to eliminate the bone-fracturing variants of BMP-2, and the bad, late-acting versions of many other genes in the human genome. This is the very reason that we age and die.
If evolution cannot help us after a certain age, why should we not help ourselves? Should not everyone have a right to the best versions of the genes in our collective genetic heritage, or at least to be born free of the worse ones?
And yet, if we reduce genetic differences, we risk turning the human population into one giant clone, tedious to meet with and bereft of the variation needed to respond to changing environments. The pursuit of perfection, if carried to extremes, is sure recipe for extinction.

 

  1. advancement of the Species

  2. selection of the characteristics of the progeny

  3. selection and isolation of certain genetic components of some disorders

  4. preservation through replication

  5. banishment of mediocrity

Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

(1) is the right choice because the question whether Genetic Engineering will ultimately result in the advancement of species or its extinction is still a controversy which in turn is the basic intention behind the passage.

Multiple choice
  1. Escherchia coli

  2. Agrobacterium tumefaciens

  3. Mycobacterium leprae

  4. Green sulphur bacteria

Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

 The basis of Agrobacterium-mediated genetic engineering is that the T-DNA of A. tumefaciens is excised and integrates into the plant genome as part of the natural infection process by this bacterium. So, any foreign DNA inserted into the T-DNA will also be integrated.

Multiple choice
  1. to kill the cancer cells

  2. for the formation of somaclonal antibodies

  3. for the formation of somatic hybrids

  4. for the formation of antibiotics

Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Monoclonal antibodies are usually produced from hybridoma clones. Each hybridoma clone is derived by the fusion of a myeloma cell and antibody producing lymphocyte.

Multiple choice
  1. crossing over

  2. emasculation

  3. asexual hybridization

  4. parasexual hybridization

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Parasexual hybridization involves the fusion of protoplasts from different species or varieties to create a hybrid, bypassing the sexual cycle.

Multiple choice
  1. E.coli

  2. Agrobacterium tumefaciens

  3. Mycobacterium leprae

  4. Green sulphur bacteria

Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Plant transformation mediated by Agrobacterium tumefaciens, a soil plant pathogenic bacterium, has become the most used method for the introduction of foreign genes into plant cells and the subsequent regeneration of transgenic plants.

Multiple choice
  1. Plastid

  2. Restriction endonucleases

  3. Heterosis

  4. Mutation

Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. They are widely used in genetic engineering.

Multiple choice
  1. providing best human food

  2. resistance to common human diseases

  3. having all important blood factors

  4. organ transplantation

Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

If the transgenic pigs carry genes for human antigens, then during organ transplantation, there will be less chances of a graft rejection by self-antibodies.

Multiple choice
  1. Escherichia coli

  2. Pseudornonas putida

  3. Pseudomonas fluorescens

  4. Trichodenna

Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Frost resistance is the ability of building materials in a wet condition to withstand many cycles of freezing and thawing without disintegrating.