Biology · Law Legal Studies
Forensic Science Techniques
654 Questions
Forensic science techniques involve the application of scientific methods like DNA fingerprinting, biometrics, and trace evidence analysis to solve crimes. These topics appear in specialized law enforcement and general science exams. Practice these questions to understand forensic fundamentals.
DNA fingerprinting methodsTrace evidence transferBiometrics and identificationForensic pathology
Forensic Science Techniques Questions
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Dactylography
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Demonology
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Gerentography
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Nomadicology
A
Correct answer
Explanation
Dactylography is the scientific study and analysis of fingerprints for identification purposes. It is derived from Greek words 'daktylos' (finger) and 'graphia' (writing). It forms the basis of forensic fingerprint analysis used in criminal investigations worldwide. The other options are unrelated: demonology (beliefs about demons), embryology (study of embryos).
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DNA supercoiling
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Nick translation
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DNA splicing
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DNA mapping
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DNA fingerprinting
E
Correct answer
Explanation
A hybridization probe is a fragment of DNA or RNA of variable length (usually 100-1000 bases long), which is used in DNA or RNA samples to detect the presence of nucleotide sequences (the DNA target) that are complementary to the sequence in the probe. The probe thereby hybridizes to single-stranded nucleic acid (DNA or RNA) whose base sequence allows probe-target base pairing due to complementarity between the probe and target.
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Terminators
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RFLPs
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VNTRs
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c DNA
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PCR
C
Correct answer
Explanation
Variable Number Tandem Repeat (or VNTR) is a location in a genome where a short nucleotide sequence is organised as a tandem repeat. DNA fingerprinting uses this sequence more frequently.
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a known gene on the same chromosome
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a homozygous individual with a simple RFLP pattern
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particular DNA sequence located on the same chromosome
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particular DNA sign co-inherited with the specific genetic trait
D
Correct answer
Explanation
Genes and anonymous DNA markers located near each other on the same chromosome are linked and do not segregate independently during meiosis.
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ABO blood type presumption
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HLA typing presumption
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DNA sequence comparison
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All will be equally accepted in the court
A
Correct answer
Explanation
Due to Bombay Phenotype and other possible exceptions, the ABO blood type system is not conclusive in determining whether or not someone could be a parent of a particular child. The other two kinds of evidence are much more reliable.
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Stephen Christmas
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William Cowper
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Richard L. Heschl
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Kurt Freund
D
Correct answer
Explanation
He was a Czech sexologist, who is famed for creating phallometry, i.e. the complete measurement of sexual arousal in males.
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Southern blotting
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Auto-radiography
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Electrophoresis
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Density gradient centrifugation
C
Correct answer
Explanation
Electrophoresis is used in DNA fingerprinting for separation of DNA fragments.
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Southern blot
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Western blot
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Northern blot
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FACS
A
Correct answer
Explanation
A southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Thus, it is used for DNA fingerprinting.
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DNA profiling
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Genome mapping
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Determination of the risk of a disease
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All of the above
D
Correct answer
Explanation
Rif-lip technique is used for DNA profiling, genome mapping and determination of the risk of a disease.
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Telomeres
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Satellites
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Centromeres
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Secondary constriction
B
Correct answer
Explanation
Satellite DNA consists of very large arrays of tandem repeats of non-coding DNA, which are the basis for DNA fingerprinting.
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To know base sequence of DNA fragment
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To detect change in the length of the restriction fragments
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To amplify smaller DNA segments
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To analyse chromosomes on basis of their shape to form a karyotype
B
Correct answer
Explanation
RFLP analysis is done to detect the change in the length of the restriction fragments.
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TaqMan-probe
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MB probe
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VNTR-probe
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LNA prime time probe
C
Correct answer
Explanation
VNTR-probe is used for the identification of tandem repeats in DNA fingerprinting.
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Difference in pattern of genes between individuals
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Difference in junk DNA pattern between individuals
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Difference in order of genes between individuals
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All of the above
B
Correct answer
Explanation
Junk DNA means non-coding DNA, which is present in the satellites. No two individuals have the same pattern of tandem repeats, i.e. junk DNA, thus it forms the basis of DNA fingerprinting.
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Restriction digestion - DNA isolation - Electrophoresis - Southern blotting - Probe hybridisation - Autoradiograph
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DNA isolation - Electrophoresis - Restriction digestion - Southern blotting - Probe hybridisation - Autoradiograph
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DNA isolation - Restriction digestion - Electrophoresis - Probe hybridisation - Southern blotting - Autoradiograph
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DNA isolation - Restriction digestion - Electrophoresis - Southern blotting - Probe hybridisation - Autoradiograph
D
Correct answer
Explanation
This is the correct sequence.
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to settle disputed parentage
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criminal identification and forensics
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personal identification
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amplification of VNTR region
D
Correct answer
Explanation
DNA fingerprinting cannot be applied to the amplification of VNTR region.