Biology · Law Legal Studies

Forensic Science Techniques

725 Questions

Forensic science techniques involve the application of scientific methods like DNA fingerprinting, biometrics, and trace evidence analysis to solve crimes. These topics appear in specialized law enforcement and general science exams. Practice these questions to understand forensic fundamentals.

DNA fingerprinting methodsTrace evidence transferBiometrics and identificationForensic pathology

Forensic Science Techniques Questions

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Process used for amplification or multiplication of DNA in DNA fingerprinting is

  1. Polymerase chain reaction

  2. Southern blotting

  3. Northern blotting

  4. None of these
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Process used for amplification or multiplication of DNA in DNA fingerprinting is Polymerase chain reaction (PCR) .It is done in invitro system.

So, the correct option is 'Option A' . 

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction is useful in

  1. Locating simple sequence repeats

  2. Locating variable number of tandem repeats

  3. Developing restriction maps

  4. Amplification of specific DNA segment.
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Polymerase chain reaction (PCR) is a method widely used in molecular biology to make many copies of a specific DNA segment. Using PCR, a single copy (or more) of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment.

So, the correct answer is 'Amplification of specific DNA segment.'

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

During PCR technique the pairing of primers to ssDNA segment is called

  1. Denaturation

  2. Annealing

  3. Polymerisation

  4. Isolation
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation
PCR - technique amplify the DNA sample in three steps namely denaturation of target DNA, annealing and polymerization. Denaturation refers to the thermal cycle that separates the DNA strands and forms the ssDNA. Annealing refers to the pairing of primers to the ssDNA and polymerisation is the extension of primer into complete DNA strand complementary to the template strand. 
So, the correct answer is 'Annealing'
Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

DNA is amplified by __________________.

  1. PCR technique

  2. Southern Blotting technique

  3. Northern Blotting technique

  4. Nucleic acid hybridization technique
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

PCR (Polymerase Chain Reaction) is the standard laboratory technique used to amplify specific segments of DNA.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerase chain reaction technique is used for.

  1. DNA amplification

  2. Screening gene libraries

  3. Synthesis of polymerase

  4. DNA sequencing
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The primary purpose of the Polymerase Chain Reaction (PCR) is to create millions of copies of a specific DNA sequence, known as DNA amplification.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following would not be a valid reason to use PCR?

  1. Help in analyzing DNA of ancient remains of fossilized woolly mammoth

  2. Help in analyzing the genome of a virus

  3. Help in determining the DNA of a hair sample found at a crime scene

  4. Help in transferring DNA from a plasmid containing GFP into E.coli
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Majorly the PCR is used in analyzing and determining purposes only not the transferring purposes. For transferring purpose vectors are used. In analyzing and determining purposes, the sample is limited i.e. if the sample gets destroyed no analysis can be carried out which can be a big problem in the investigation cases. So to have extra copies of the sample it is first amplified by PCR.

Multiple choice organization of commerce and management employee selection process meaning and steps in selection meaning, importance, methods and procedure of selection steps in employee selection process

_________ test examine the subject's response whether it is truthful or deceptive by examining the biological movements recorded on the paper.

  1. Polygraph test

  2. Graphology test

  3. Personality test

  4. Integrity test
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

A polygraph test records physiological indicators like heart rate and skin conductivity to determine if a subject is being truthful or deceptive.

Multiple choice

What is the purpose of Gel electrophoresis?

  1. Laboratory technique used to separate fragments of DNA

  2. Used to create multiple copies of a DNA sample

  3. Comparison of different DNA

  4. Single-stranded chromosomes
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Gel electrophoresis is a laboratory technique used to separate DNA fragments based on their size and charge as they move through a gel matrix under an electric field.

Multiple choice

What is the first step for DNA profiling?

  1. A DNA sample is collected and then amplified using PCR

  2. Satellite DNA are cut with to generate fragments

  3. Fragment length will differ due to the variable length

  4. The fragments are separated using gel electrophoresis
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The first step in DNA profiling is obtaining a sample of DNA and amplifying the specific regions of interest using Polymerase Chain Reaction (PCR) to get enough material for analysis.

Multiple choice

Which is a use for DNA profiling?

  1. Determination of a familial relationship

  2. Identification of a crime suspect

  3. Both
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

DNA profiling is used for both forensic identification of suspects and for determining biological relationships, such as paternity testing.

Multiple choice

What is the purpose of PCR (Polymerase chain reaction)?

  1. Make copies of a DNA sample

  2. Destroy a DNA sample

  3. Preserve a DNA sample
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify a specific segment of DNA, effectively making millions of copies from a small sample.

Multiple choice botany cell and cellular organization cell theory microscope cell and its discovery

A fluorescent microscope is used

  1. For increasing resolving power.

  2. For obtaining X-ray diffraction pattern of DNA.

  3. To study cell and their components in a living state.

  4. To localise cell structure stained with fluorochrome dyes.
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of or in addition to, reflection and absorption to study properties of organic or inorganic substances. The "fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescent image. So, fluorescent microscope is used to localise cell structure stained with fluorochrome dyes and not for increasing resolving power, for obtaining X-ray diffraction pattern of DNA and to study cell and their components in a living state.