The factor that is least important to control in order to limit food-borne illness is
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Presence of pesticides
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Presence of microbes
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Temperature of food
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Time of incubation
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None of the above
Foodborne illness can be caused by pesticides or medicines in food and naturally toxic substances such as poisonous mushrooms or reef fish. Foodborne illnesses may result from the consumption of food contaminated by microbial pathogens, toxic chemicals or radioactive materials. The temperature range in which food-borne bacteria can grow is known as the danger zone. Food safety agencies, such as the United States' Food Safety and Inspection Service (FSIS), define the danger zone as roughly 4–5°C to 60 °C. The FSIS stipulates that potentially hazardous food should not be stored at temperatures in this range in order to prevent foodborne illness. The delay between consumption of a contaminated food and appearance of the first symptoms of illness is called the incubation period. This ranges from hours to days, depending on the agent, and on how much was consumed.