Biomolecules and Enzymes

Paratope is the portion of antibody, which binds with antigen.

Affinity chromatography using Nickel can be used to separate protein tagged by histidine.

Eastern blotting is a technique to detect protein post translational modification and is an extension of the biochemical technique of western blotting.

Non-standard amino acids refer to those amino acids that have been chemically modified after they have been incorporated into a protein and those amino acids that occur in living organisms but are not found in proteins.

Porphyrins are a group of chemicals that help to form many important substances in the body including haemoglobin, which is the protein in red blood cells that carries oxygen in the blood. The standard amino acid glycine is used to synthesise porphyrins used in red blood cells.

Water accounts for 92% of plasma.

Glycogen is not present in plasma. It is storage form of glucose.

This option is correct as self assembly of lipid layer membrane in cells of living systems is an example of mesoscale self assembly which is caused by the capillary interaction between individual units.

This is not true as enzymes are never used up in the reaction. This is the main feature of enzyme working.

Deamination is the removal of an amine group from a molecule. This occurs in liver.

Albumin, globulin and fibrogen are serum proteins. They are also known as blood plasma proteins.

B-hemolytic streptococci are classified on the basis of group-specific C carbohydrate antigen on cell wall into 20 lancefield groups (A To V except I and J). Group A streptococci are further classified by serological typing of (M) proteins into Griffith types.

The plasma protein and their fractions can be separated by electrophoretic method,  which depends upon electric charge of the proteins and difference in their rate of migration. Maximum mobility in electrophoresis is shown by albumin and minimum by y-globulin.

Each N-acetylmuramic acid is attached to a short amino acid chain containing L-alanine, D- glutamine, L-lysine and D-alanine with a pentaglycine interbridge between tetrapeptides in the case of Staphylococcus aureus, a gram positive bacterium. Meso-diaminopimelic acid serves as the final precursor in the biosynthesis of L-lysine in both bacteria and higher plants, and is also an essential component of the peptidoglycan of most pathogenic bacteria, nearly all gram-negative and most gram-positive bacteria with the notable exception of Staphylococcus aureus where it is replaced by lysine.

The human body's own antibiotic lysozymes found in tears work by breaking the β(1, 4) glycosidic bonds in peptidoglycan, and thereby destroying many bacterial cells.