Multiple choice The first committed step in the Glycolytic pathway is formation of ... Glucose 6-P Fructose 6-P Fructose 1,6-Bisphosphate PGAL Reveal answer Fill a bubble to check yourself C Correct answer Explanation The conversion of Fructose 6-phosphate to Fructose 1,6-bisphosphate by PFK-1 is the rate-limiting and first committed step of glycolysis.