Multiple choice Which of the following is the first step of glycolysis? Breakdown of glucose Phosphorylation of glucose Conversion of glucose into fructose Dehydrogenation of glucose - Reveal answer Fill a bubble to check yourself B Correct answer Explanation In the first step of glycolysis, the glucose ring is phosphorylated. Phosphorylation is the process of adding a phosphate group to a molecule that is derived from ATP. As a result, at this point in glycolysis, one molecule of ATP is consumed.