Multiple choice Which is the first step of glycolysis? Cleavage of Fructose-1,6-diphosphate Phosphorylation of glucose Oxidative dehydrogenation Isomerisation Dehydration Reveal answer Fill a bubble to check yourself B Correct answer Explanation Glycolysis starts with the phosphorylation of glucose in G-6-P by using two ATP molecules in the presence of hexokinase enzyme.