Multiple choice Transcription occurs along a __template forming an mRNA in the __ direction 5' to 3'; 5' to 3' 5' to 3'; 3' to 5' 3' to 5'; 5' to 3' 3' to 5'; 3' to 5' Reveal answer Fill a bubble to check yourself C Correct answer Explanation During transcription, RNA polymerase reads the template DNA strand in the 3' to 5' direction to synthesize a complementary mRNA strand in the 5' to 3' direction.