Multiple choice What role does DNA gyrase play in DNA replication? Breaks covalent bonds between DNA nucleotides Separates nitrogenous bases by breaking hydrogen bonds Supercoils the DNA in preparation for DNA replication Relieves strain + prevents supercoiling of separated strands Reveal answer Fill a bubble to check yourself D Correct answer Explanation DNA gyrase (a type of topoisomerase) relieves the torsional strain (supercoiling) that builds up ahead of the replication fork as helicase unwinds the DNA.