Multiple choice

What is the purpose of using Mn2+ (divalent cations) in Polymerase Chain Reaction (PCR ) instead of Mg2+?

  1. To orientate dNTP within the active centre

  2. To impart positive charge to dNTP

  3. To increase the error rate

  4. To increase the accuracy rate

  5. None of these
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

As higher Mn2+ concentration increases the error rate during DNA synthesis, Mn2+can be utilized for PCR-mediated DNA mutagenesis.

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