Multiple choice

PCR requires

  1. Li++

  2. Ca++

  3. Na++

  4. Mg++
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

PCR (Polymerase Chain Reaction) requires Mg++ ions as an essential cofactor for Taq polymerase and other DNA polymerases used in PCR. Mg2+ is required for enzyme activity and stabilizes the DNA duplex. The reaction buffer contains MgCl2. Lithium, calcium, and sodium ions are not required for PCR - in fact, calcium can be inhibitory and the others are not part of the standard reaction mix.

Keep practicing — related questions