Multiple choice

PCR requires:

  1. Li++

  2. Ca++

  3. Na++

  4. Mg++
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

PCR requires magnesium ions (Mg2+) as essential cofactors for Taq polymerase activity. Mg2+ stabilizes the DNA duplex and is required for the polymerase to function properly. The concentration of Mg2+ also affects primer annealing and specificity. Lithium, calcium, and sodium are not required cofactors for PCR.

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