Multiple choice

Which of the following statements is true for Gram negative organisms?

  1. Due to presence of higher lipid content, after alcohol-treatment, the porosity of the cell wall increases, hence the CVI complex (Crystal violet -Iodine) can pass through. Thus, the primary stain is not retained.

  2. Due to presence of rich level of peptidoglycan, after alcohol-treatment, the porosity of the cell wall increases, hence the CVI complex (Crystal violet -Iodine) can pass through. Thus, the primary stain is not retained.

  3. Primary stain retain after counter stain, violet or purple colour in Gram negative.

  4. Gram negative organisms lacks the secondary membrane and lipopolysaccharide.

  5. Gram negative organisms have teichoic acids or lipoteichoic acids.
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

True statement: Gram negative has higher lipid content, after alcohol-treatment, the porosity of the cell wall increases, hence the CVI complex (Crystal violet -Iodine) can pass through. Thus, the primary stain is not retained. It become red or pink in colour and can differentiate between Gram negative and Gram positive organisms.

Keep practicing — related questions