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Microbiology - Cell Staining Methods and Techniques

Covers cell staining methods and techniques in microbiology, including Gram staining, acid-fast staining, supravital stains, and various staining applications.

14 Questions Published
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Questions

Question 1 Multiple Choice (Single Answer)

Gram staining is a method of

  1. negative staining
  2. acid fast staining
  3. differential staining
  4. endospore staining
  5. simple staining
Question 2 Multiple Choice (Single Answer)

Which of the following is/are Supravital stains?

  1. New Methylene Blue for reticulocyte staining
  2. Brilliant Cresyl Blue for reticulocyte staining
  3. Trypan blue for eukaryotic cells
  4. Propidium iodide for eukaryotic cells
  5. Both the options 1 and 2
Question 3 Multiple Choice (Single Answer)

To achieve desired effects, the stains are used in very dilute solutions

  1. ranging from 1:100 to 1:000
  2. ranging from 1:10 to 1:100
  3. ranging from 1:5000 to 1:500000
  4. ranging from 1:2500 to 1:4000
  5. ratio should be 1:1
Question 4 Multiple Choice (Single Answer)

Which of the following is/are a decolouriser in Gram staining?

  1. Acetone and Ethanol
  2. Crystal violet
  3. Iodine
  4. Fuchsin and safranin
  5. Carbol fuchsin
Question 5 Multiple Choice (Single Answer)

Which of the following is not used in standard procedure of Gram staining?

  1. Iodine
  2. Carbol fuchsin
  3. Acetone and Ethanol
  4. Fuchsin and safranin
  5. Crystal violet
Question 6 Multiple Choice (Single Answer)

Which of the following statements is true?

  1. Gram positive become red after counter staining.
  2. Gram negative becomes purple after counter staining.
  3. Gram negative organisms lack secondary membranes and lipopolysaccharide.
  4. Gram positive organisms lack the secondary membrane and lipopolysaccharide.
  5. Gram positive organisms have the secondary membrane and lipopolysaccharide.
Question 7 Multiple Choice (Single Answer)

Chelating agents, for certain types of adherence, are found in

  1. Peptidoglycan, found in both Gram positive and negative organisms.
  2. Lipopolysaccharide are found in Gram positive organisms.
  3. Lipopolysaccharide are found in Gram negative organisms.
  4. Teichoic and lipoteichoic acids are found in Gram positive organisms.
  5. Teichoic and lipoteichoic acids are found in Gram negative organisms.
Question 8 Multiple Choice (Single Answer)

Which of the following staining is used in the disease Diphtheria to demonstrate the presence or absence of metachromatic granules?

  1. Gram staining
  2. Negative staining
  3. Albert’s staining
  4. Both Gram staining and Albert’s staining
  5. Silver staining
Question 9 Multiple Choice (Single Answer)

Which of the following is/are Acid Fast method?

  1. Kinyoun stain
  2. Ziehl–Neelsen stain
  3. Fite stain
  4. Auramine-rhodamine stain
  5. All of the above
Question 10 Multiple Choice (Single Answer)

Which of the following statement is FALSE?

  1. Gram staining was invented in the year 1882.
  2. Iodine is used as a mordant in Gram staining.
  3. Gram positive and Gram negative differential helps to take the appropriate antibiotic treatment.
  4. Crystal violet is an anionic charged dye and the exterior of cells have cationic charge.
  5. Aliquot something means “Take a smaller volume of sample.”
Question 11 Multiple Choice (Single Answer)

Which of the following is not a modified method of Gram Staining?

  1. Auramine and Rhodamine
  2. Jensen’s
  3. Kopeloff and Beerman’s
  4. Preston and Morrell’s
  5. Weihert’s
Question 12 Multiple Choice (Single Answer)

Nigrosin, is used for

  1. gram staining
  2. negative staining
  3. Ziehl-Neelsen staining
  4. Papanicolaou staining
  5. silver staining
Question 13 Multiple Choice (Single Answer)

Which Statement is TRUE about Haematoxylin and Eosin (H&E) staining in Histology of the lung?

  1. Haemotoxylin stains both cell nuclei and red blood cells in red.
  2. Eosin stains both cell nuclei and red blood cells in pink, or bright red.
  3. Haematoxylin stains cell nuclei blue, while Eosin stains red blood cells bright red.
  4. Haematoxylin stains red blood cells in bright red, while Eosin stains cell nuclei blue.
  5. Haemotoxylin and Eosin stains only red blood cells.
Question 14 Multiple Choice (Single Answer)

Which of the following statements is true for Gram negative organisms?

  1. Due to presence of higher lipid content, after alcohol-treatment, the porosity of the cell wall increases, hence the CVI complex (Crystal violet -Iodine) can pass through. Thus, the primary stain is not retained.
  2. Due to presence of rich level of peptidoglycan, after alcohol-treatment, the porosity of the cell wall increases, hence the CVI complex (Crystal violet -Iodine) can pass through. Thus, the primary stain is not retained.
  3. Primary stain retain after counter stain, violet or purple colour in Gram negative.
  4. Gram negative organisms lacks the secondary membrane and lipopolysaccharide.
  5. Gram negative organisms have teichoic acids or lipoteichoic acids.
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