Multiple choice

Which of the given structures has a role of permeability barrier in preventing the loss of the primary stain crystal violet in gram staining procedure?

  1. Peptidoglycan

  2. Lipopolysaccharide

  3. Outer membrane

  4. Teichoic acids

  5. Core polysaccharide
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A Correct answer
Explanation

Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet. Gram negative bacteria has a thinner peptidoglycan wall, which does not retain the crystal violet during the decolouring process.

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