Multiple choice

Which of the following is the correct sequence of procedures for interspecific somatic hybridization in plants?

  1. Procuring haploid cells from the parental species à isolation of protoplast by removing the cellulose wall à protoplast fusion à regeneration of fused cell

  2. Isolation of somatic cells à extraction of protoplast à protoplast fusion à cutting of the fused cell

  3. Isolation of haploid and somatic cells à extraction of cells

  4. Enzymatic isolation of haploid cellà isolation of protoplast fusion of extracted protoplasts à regeneration of the fused cell
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A Correct answer
Explanation

Interspecific somatic hybridization requires: (1) obtaining haploid cells from parental species, (2) removing cellulose walls to isolate protoplasts using enzymes like cellulase and pectinase, (3) fusing protoplasts using PEG or electrofusion, and (4) regenerating the fused hybrid cells into plants through tissue culture. Option A correctly sequences these steps.

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