Multiple choice

The first step in the technique of protoplasmic fusion is the

  1. hybridization

  2. collection of somatic cells

  3. isolation of protoplasts

  4. selection and isolation of somatic cells
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C Correct answer
Explanation

Protoplast fusion requires first removing the cell wall to isolate protoplasts (naked plant cells). Only after isolation can protoplasts be fused using techniques like PEG or electrofusion. Hybridization is the goal, not the first step, and somatic cell collection precedes protoplast isolation.

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