Multiple choice Which of the following statements is correct concerning Michales constant Km? It is measured in the same units as the rate of the enzyme-catalysed reaction. It varies with the amount of enzyme used in measuring the reaction rate. It can be considered to be a measure of affinity of an enzyme for its substrate. It has a low value to indicate a low affinity for the substrate. Reveal answer Fill a bubble to check yourself C Correct answer Explanation The concentration of substrate when the velocity of the reaction is half of the maximum velocity is expressed as Michales Menten constant Km. The lower the value of Km, the greater the affinity of enzymes for the substrate.