Multiple choice

If Kcat of an enzyme is 30 sec-1, the Michalis Menton constant is 5 x 10-3 and the total enzyme concentration is 15μg, what is the maximum velocity of the reaction?

  1. 45 mg/min

  2. 2 x 103 mg/min

  3. 4.5 mg/min

  4. 0.5 mg/min

  5. 30 mg/min

Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Kcat = Vmax/Total Enzyme concentration. Vmax = Kcat* total enzyme concentration = 30*15*10-3 mg/min = 45 mg/min.