Tag: tissue culture

Questions Related to tissue culture

Banana can be rapidly multiplied by ____________.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Multiple shoot production

  2. Embryoid formation

  3. Shoot tip culture

  4. Callus formation

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Correct Option: A

Who is known as father of plant tissue culture?

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Haberlandt

  2. Steward

  3. White

  4. Skoog.

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Correct Option: A

Haploids are useful for ________________.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Production of callus

  2. Production of homozygous diploids

  3. Microdensitometer studies

  4. Induction of giant cells

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Correct Option: A

Which of the following statements are correct?
a. Callus is the disorganised mass of cells formed under in vitro condition.
b. Totipotency is the ability of a plant organ to regenerate an entire organism when cultured in a suitable medium.
c. Embryoid is the embryo-like structure formed under in vitro condition which has the potentiality to develop into a plantlet.
d. Explant is the excised portion of plant tissue or organ which is used in tissue culture study.
Select the correct answer using the codes given Codes.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. $1, 2$ and $4$

  2. $1, 3$ and $4$

  3. $1, 2$ and $3$

  4. $2, 3$ and $4$

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Correct Option: A

The callus is defined as ________________.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Decaying cells

  2. Differentiated cells

  3. Sterile mass of cells

  4. Undifferentiated mass of cells

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Correct Option: A

Electroporation is a technique commonly used in ________________.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Tissue culture

  2. Somatic hybridization

  3. Radioimmuno assay

  4. Electrophoresis

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Correct Option: A

Consider the following statements.
Tissue culture is recommended for.
a. Multiplication of elite genotypes
b. Developing virus-free plants
c. Production of secondary metabolites
d. Induction of polyploidy
e. Of useful trees
Of this statements.

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. $1, 2$ and $4$ are correct

  2. $1, 2$ and $3$ are correct

  3. $1, 3$ and $4$ are correct

  4. $2, 3$ and $4$ are correct

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Correct Option: A

Somaclones are obtained by : -

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Genetic engineering

  2. Tissue culture

  3. Plant breeding

  4. Irradiation Genetic engineering

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Correct Option: B

Which of the following is not a common procedure in tissue culture of plants?

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Shoot tip culture for the purpose of micropropagation

  2. Leaf, stem, and root culture for the purpose of cell suspension cultures

  3. Protoplast culture for the purpose of genetic engineering of plants

  4. Culture of hybridized mature plant cells

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Correct Option: D
Explanation:

A) Shoot tip culture may be described as the culture of terminal portion of a shoot comprising the meristem together with primordial and developing leaves and adjacent stem tissue. It is a common process used for micropropagation

B) Suspension culture is a type of culture in which single cell or small aggregates of cells mul­tiply while suspended in agitated liquid medium. It is the process done in tissue culture using plant part such as leaf, stem, roots etc.
C) Protoplasts are naked plant cells without the cell wall, but they possess plasma membrane and all other cellular components. Protoplasts of different species can be fused to generate a hybrid and this process is referred to as somatic hybridization. Genetic transformations can be achieved through genetic engineering of protoplast DNA
D) After hybridization or suspension culture, a new mature plant is produced. This plant is identical to its parent plant
So, the correct answer is 'culture of hybridized mature plant cells'

Agitation during tissue culture serves for

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Aeration of the culture

  2. Constant Mixing

  3. Breakage of cell aggregates in small particles

  4. All of the above

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Correct Option: D
Explanation:

Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation. Agitation during tissue culture serves for:

  • Aeration of the culture
  • Constant Mixing
  • Breakage of cell aggregates in small particles