Tag: tissue culture

Quizzes (2) Questions (86)

Questions Related to tissue culture

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Banana can be rapidly multiplied by ____________.

  1. Multiple shoot production

  2. Embryoid formation

  3. Shoot tip culture

  4. Callus formation
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Banana is commercially and rapidly multiplied in vitro primarily through multiple shoot production, often initiated from shoot tip cultures. This method allows for the production of many genetically identical plantlets.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Haploids are useful for ________________.

  1. Production of callus

  2. Production of homozygous diploids

  3. Microdensitometer studies

  4. Induction of giant cells
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Haploid plant cultures are primarily used for generating homozygous doubled haploids through chromosome doubling, which rapidly produces pure lines for breeding. Callus is formed from explants rather than being a specific use of haploids, and the other options do not relate to haploid utility.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Which of the following statements are correct?
a. Callus is the disorganised mass of cells formed under in vitro condition.
b. Totipotency is the ability of a plant organ to regenerate an entire organism when cultured in a suitable medium.
c. Embryoid is the embryo-like structure formed under in vitro condition which has the potentiality to develop into a plantlet.
d. Explant is the excised portion of plant tissue or organ which is used in tissue culture study.
Select the correct answer using the codes given Codes.

  1. $1, 2$ and $4$
  2. $1, 3$ and $4$
  3. $1, 2$ and $3$
  4. $2, 3$ and $4$
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Statements 1, 3, and 4 correctly define callus as a disorganised mass of cells, embryoid as an embryo-like structure formed in vitro, and explant as the excised plant tissue. Statement 2 is slightly imprecise or often excluded in specific code selections because totipotency refers to the genetic potential of a cell rather than just an organ.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

The callus is defined as ________________.

  1. Decaying cells

  2. Differentiated cells

  3. Sterile mass of cells

  4. Undifferentiated mass of cells
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

A callus is an unorganized and undifferentiated mass of proliferating plant cells formed when an explant is cultured in vitro. Differentiated cells form specific tissues, while decaying cells refer to dead matter.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Electroporation is a technique commonly used in ________________.

  1. Tissue culture

  2. Somatic hybridization

  3. Radioimmuno assay

  4. Electrophoresis
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Electroporation is a technique used to introduce foreign DNA into cells by applying a high-voltage electrical pulse to create temporary pores in the cell membrane. It is a standard method in plant and animal tissue culture for transformation.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Consider the following statements.
Tissue culture is recommended for.
a. Multiplication of elite genotypes
b. Developing virus-free plants
c. Production of secondary metabolites
d. Induction of polyploidy
e. Of useful trees
Of this statements.

  1. $1, 2$ and $4$ are correct
  2. $1, 2$ and $3$ are correct
  3. $1, 3$ and $4$ are correct
  4. $2, 3$ and $4$ are correct
Reveal answer Fill a bubble to check yourself
A Correct answer
Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Which of the following is not a common procedure in tissue culture of plants?

  1. Shoot tip culture for the purpose of micropropagation

  2. Leaf, stem, and root culture for the purpose of cell suspension cultures

  3. Protoplast culture for the purpose of genetic engineering of plants

  4. Culture of hybridized mature plant cells
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

A) Shoot tip culture may be described as the culture of terminal portion of a shoot comprising the meristem together with primordial and developing leaves and adjacent stem tissue. It is a common process used for micropropagation

B) Suspension culture is a type of culture in which single cell or small aggregates of cells mul­tiply while suspended in agitated liquid medium. It is the process done in tissue culture using plant part such as leaf, stem, roots etc.
C) Protoplasts are naked plant cells without the cell wall, but they possess plasma membrane and all other cellular components. Protoplasts of different species can be fused to generate a hybrid and this process is referred to as somatic hybridization. Genetic transformations can be achieved through genetic engineering of protoplast DNA
D) After hybridization or suspension culture, a new mature plant is produced. This plant is identical to its parent plant
So, the correct answer is 'culture of hybridized mature plant cells'

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Agitation during tissue culture serves for

  1. Aeration of the culture

  2. Constant Mixing

  3. Breakage of cell aggregates in small particles

  4. All of the above
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation. Agitation during tissue culture serves for:

  • Aeration of the culture
  • Constant Mixing
  • Breakage of cell aggregates in small particles