Tag: tissue culture

Questions Related to tissue culture

Callus is

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Undifferentiated mass of a tissue

  2. Root formation in culture media

  3. Plant hormones

  4. Plant byproduct

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Correct Option: A
Explanation:

Plant callus is a mass of unorganized cells derived from plant tissue for use in biological research and biotechnology. In plant biology, callus cells are those cells that cover a plant wound. Callus formation is induced from plant tissues after surface sterilization and plating onto in vitro tissue culture medium. Plant growth regulators, such as auxins, cytokinins and gibberellins are supplemented into the medium to initiate callus formation or somatic embryogenesis.

So, the correct answer is 'underdifferentiated mass of a tissue'

Cryobiology deals with

biology strategies for enhancement in food production common modes of vegetative propagation plant breeding for food production tissue culture

  1. Temperature effect

  2. Physiology

  3. Anatomy

  4. Characteristics of biomolecule

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Correct Option: A
Explanation:
Physiology is the scientific study of normal function in living systems. 
Anatomy is the branch of biology concerned with the study of the structure of organisms and their parts.

Cryobiology is the branch of biology that studies the effects of low temperatures on living things within Earth's cryosphere or in science. 
So, the correct answer is 'temperature effect'

Piece of sterile plant tissue that is used for tissue culture under aseptic condition is

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Inoculant

  2. Explant

  3. Clone

  4. Somaclone

Show answer
Correct Option: B
Explanation:

Explant is any portion taken from a plant that will be used to initiate a culture. It can be a portion of the shoot or of the leaves or even just some cells. It is a piece of sterile plant tissue to be used for tissue culture under aseptic conditions. Inoculant is the active material used in an inoculation. Clones are organisms that are exact genetic copies. Somacloning is cloning of plant with a single cell. 

Thus, the correct answer is option B.

Callus was grown successfully for the first time by

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. White

  2. Gautheret

  3. Nobecourt

  4. All of the above

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Correct Option: D

The study of plant and animal tissues outside the body (in a glass tube) is called

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. In vivo

  2. In vitro

  3. Innate

  4. Ecdysis

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Correct Option: B

A plant cell has potential to develop into full plant. The property is called

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Tissue culture

  2. Pleuripotency

  3. Totipotency

  4. Gene cloning

Show answer
Correct Option: C
Explanation:
  • Totipotency is the ability of a single cell to divide and produce all of the differentiated cells in an organism. 
  • For example, Spores and zygotes are examples of totipotent cells.
  • Hence a plant cell has the potential to develop into a full plant. The property is called Totipotency.
  • So, the correct answer is 'Totipotency'.

Concept of cellular totipotency was established by

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. White

  2. Haberlandt

  3. Steward

  4. Muir

Show answer
Correct Option: B
Explanation:
  • Cellular totipotency is the ability of a single cell to produce all cell types and to organize them into an entire organism when cultured in a suitable culture medium at an appropriate temperature and aeration conditions. For example, Spores and zygote are examples of the totipotent cell.
  • The concept of totipotency was established by a German Botanist, Professor Gottlieb Haberlandt in 1902 in the plant body. He stated that every living cell of the plant body able to regenerate the whole plant body because it is derived from the fertilized egg and contain hereditary information.
  • Hence concept of cellular totipotency was established by Haberlandt.
  • So, the correct answer is 'Haberlandt'.

In which year did White perform successful tissue culture?

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. 1989

  2. 1932

  3. 1929

  4. 1922

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Correct Option: B

Who proposed for the first time that cells are totipotent?

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. White

  2. Haberlandt

  3. Steward

  4. Halperin and Wetherell

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Correct Option: B

Steward et. al., performed experiment to prove cellular totipotency on

botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

  1. Tomato

  2. Carrot

  3. Tobacco

  4. Potato

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Correct Option: B