Tag: plant tissue culture

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Questions Related to plant tissue culture

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Cloning is obtained through

  1. Hybridisation

  2. Cross-pollination

  3. Self-pollination

  4. Micropropagation
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Micropropagation is the method of large production of plantlets in a less time. In this method, a small piece of plant tissue (explant) is cultured in a sterile medium which later develops into new plantlets. The offsprings produced by this methods are the exact copy of their parent plant and are known as somaclones. So, the technique of micropropagation is also known as cloning.

Thus, the correct answer is option D. 

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

What is micropropagation?  

  1. Germination of seed with cotyledons above the soil.

  2. A technique to obtain new plants by cultivating the cells or tissues in culture medium.

  3. The mature stage of endosperm.

  4. To manufacture hormones.
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Micropropagation is a technique to obtain new plants by cultivating the cell or tissue in culture medium through tissue culture.

So, the correct answer is 'A technique to obtain new plants by cultivating the cells or tissues in culture medium'

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Most plant tissue cultures are initiated from

  1. Callus

  2. Explants

  3. Plantlets

  4. Protoplasts

  5. Anthers
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

The tissue obtained from a plant to be cultured is called an explant. Explants can be taken from different many parts of a plant, including portions of shoots, leaves, stems, flowers, roots, single undifferentiated cells and from many types of mature cells provided are they still contain living cytoplasm and nuclei and are able de-differentiate and resume cell division. This has given rise to the concept of totipotentency of plant cells. So, the correct answer is 'Explants'.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Callus culture is useful for purposes like

  1. It helps in the production of secondary plant products.

  2. It is useful for the synthesis of starting compounds that are subsequently modified to yield the desired product.

  3. It is the starting materials for vegetative propagation of plants.

  4. All of the above
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation
Callus culture is useful for many purposes:
1  Callus is the starting material for the suspension culture which cells are separated.
2. It helps in the production of secondary plant products.
3. It is useful for the synthesis of starting compounds that are subsequently modified to yield the desired product.
4. It is the starting materials for vegetative propagation of plants.
Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

In plant tissue culture, undifferentiated tissue is referred to as

  1. Callus

  2. Bark

  3. Xylem

  4. None of the above
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

In plant tissue culture, undifferentiated tissue is referred to as callus, although a callus can contain meristematic nodules that may not be obvious to the naked eye but which never develop further unless suitable conditions are supplied.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Reproducing plants by cells instead of seeds is called as

  1. Tissue culture

  2. Amphimixis

  3. Mutation

  4. None of the above
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

Tissue culture is a process that involves exposing plant tissue to a specific regimen of nutrients, hormones, and light under sterile, in vitro conditions to produce many new plants, each a clone of the original mother plant, over a very short period of time. The term plant tissue culture (micro propagation) is generally used for the aseptic culture of cells, tissues, organs and their components under defined chemical and physical conditions in vitro.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

The technique of producing large number of genetically similar plants within short time by tissue culture is called as _____________.

  1. Organogenesis

  2. Somatic hybridization

  3. Micro-propagation

  4. Protoplast culture
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

Micro-propagation means the propagation of plants by growing plantlets in tissue culture and then planting them out.

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Rapidly dividing unorganised mass of cells in tissue culture is

  1. Callose.

  2. Callus.

  3. Embryoid.

  4. Plantlet.
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

Tissue culture is a growth of cells or tissues separate from the organism in an artificial environment. The process is called as micropropagation in plant tissue culture. In tissue culture, the small piece of plant or animal is used to grow complete individual and those small parts are called as explants whereas complete grown individuals are called clones. The explant initially turns into rapidly dividing unorganised mass of cells on solid or liquid culture and that is called a callus. 

Multiple choice botany tissue culture common modes of vegetative propagation plant breeding for food production plant tissue culture

Tissue culture was developed by

  1. Haberlandt

  2. Laibach

  3. White

  4. Steward
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

In 1902 C.Haberlant was the first attempted to culture isolated plant cells invitro on artificial medium. But the discovery of the use of auxin is done by three scientists Gautherel, Nobecourt, and White. This discovery is the second phase of plant tissue culture. Then White discovery's proves that plant could be successfully regenerated from undifferentiated tissue or even single cell in culture called tissue culture. Then tissue culture was developed by P R White in 1925.
So the correct option is C.