Tag: biotechnological regulations

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Questions Related to biotechnological regulations

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Polymerisation of DNA is in

  1. $3' \rightarrow 5'$ direction
  2. $5' \rightarrow 3'$ direction
  3. Both $3' \rightarrow 5'$ and $5' \rightarrow 3'$ direction

  4. None of the above
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation
DNA polymerase has 5' $\rightarrow$ 3' activity. All known DNA replication systems require a free 3' hydroxyl group before synthesis can be initiated (note: the DNA temple is read in 3' to 5' direction whereas a new strand is synthesized in 5' to 3' direction).
So, the correct answer is "5' $\rightarrow$ 3' ".
Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

In PCR, short primers are added to single stranded DNA molecules in a test tube and the appropriate enzymes are included to make a copy of the DNA. Which of the following primers is needed for copying the single stranded DNA sequence $ 5'TACGGTAGGTC3'$ ?

  1. $5'ATGCC 3'$
  2. $ 5'GACCT 3' $
  3. $ 3'GACCT 5' $
  4. $ 3'ATGCC 5' $
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

A primer is a short single strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3′-end of the primer, and copies the opposite strand. 


For copying the single stranded DNA sequence 5′TACGGTAGGTC3′, complementary sequence possessing primer is needed which is 5′GACCT3′.

So the correct option is '5′GACCT3′'.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

A DNA sequencing reaction was performed with the fragment $5-XXXGCGATCGYYYY-3'$ as the template, dideoxy GTP, all the four $dNTPs$, and the required primers and enzyme. $XXXX$ and $YYYY$ in the given DNA fragment represent primer binding sites. The set of fragments obtained during the reaction will be (the primers are not shown in the amplified fragments).

  1. $5'-CGATCGC-3'$ only
  2. $5'-CG-3', 5'-CGCTAG-3', 5'-CCCTAGC-3'$
  3. $5'-CG-3', 5'-CGATCG-3', 5'-CGATCGC-3'$
  4. $5'-G-3', 5'-GCG-3', 5'-GCGATCG-3'$
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

A DNA sequencing reaction was performed with the fragment 5−XXXGCGATCGYYYY−3′ as the template, dideoxy GTP, all the four dNTPs, and the required primers and enzyme. XXXX and YYYY in the given DNA fragment represent primer binding sites. The set of fragments obtained during the reaction will be CGATCGC, because if primer is attache to the XXXX binding site it polymerization starts and its complimentary fragment is CGATCGC.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Column I                                            Column II
I. Agarose                                             A. PCR
II. Opines                                              B. Gene gun
III. Biolistic                                           C. Ti plasmid
iv. Thermal cycler                                 D. Sea weeds

  1. $I-D,II-A,III-B,IV-C$
  2. $I-D,II-C,III-B,IV-A$
  3. $I-D,II-A,III-C,IV-B$
  4. $I-A,II-D,III-B,IV-C$
Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

  • Agarose is derived from seaweeds Gelidium and Gracilaria
  • Opine synthesis is the function of Ti plasmid of Agrobacterium
  • Biolistic is the term used for gene gun used for biotransformation
  • Thermocycler is used to carry out PCR reaction
So the correct option is B. (I−D,II−C,III−B,IV−A)

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

The areas of application of PCR include

A. Production of monoclonal antibodies 
B. Insertion of recombinant DNA into an organism
C. Diagnosis of specific mutation
D. Detection of plant pathogens

  1. A, B

  2. B, C

  3. C, D

  4. A, B, C, D
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

The areas of application of PCR include 

  • Clinical Diagnosis
  • DNA Sequencing 
  • Gene Manipulation and Expression Studies
  •  in Comparative Studies of Genomes
  •  in Forensic Medicine
  •  in Comparison with Gene Cloning


So, the correct option is 'C,D'.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following can't be achieved using PCR ?

  1. Detect HIV in AIDS suspect

  2. detect mutations in cancer patients

  3. Detect antigen - antibody interaction

  4. Detect specific micro organism from soil
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

PCR detects genetic material (DNA/RNA). Antigen-antibody interactions are detected using techniques like ELISA, not PCR.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

First technique and product produced by biotechnology are:

  1. PCR and detection of disease

  2. ELISA and detection of AIDS

  3. PCR and medicines

  4. Fermentation and Ethanol
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

While fermentation is an ancient biotechnological process, in the context of modern molecular biotechnology, PCR and its application in disease detection are often cited as foundational developments.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which of the following has popularised the PCR(Polymerase chain reactions)?

  1. Easy availability of DNA template

  2. Availability of synthesis primers

  3. Availability of cheap deoxyribonucleotides

  4. Availability of thermostable DNA polymerase
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Polymerase chain reaction (PCR) relies on repeated cycles of heating and cooling for denaturation and annealing, which would normally destroy standard enzymes. The breakthrough that popularized PCR was the discovery of thermostable DNA polymerase, such as Taq polymerase, which can withstand high temperatures without denaturing.

Multiple choice bio-chemistry applications of biotechnology pcr cutting, insertion and ligation of dna gene cloning procedure biotechnological regulations asexual modes of reproduction

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

  1. $5$' ______ GAATTC ________$3$

    $3$' _______ CTTAAG __________$5$'
  2. $5$' __________CACGTA _________ $3$'

    $3$'________ CTCAGT ________ $5$'
  3. $5$' ________ CGTTCG ________ $3$'

    $3$' _________ATGGTA _________$5$'
  4. $5$' _________ GATATG _________$3$'

    $3$'________CTACTA ________$5$'
Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

The sequence 5'-GAATTC-3' paired with 3'-CTTAAG-5' is the classic palindromic recognition site for the restriction enzyme EcoRI, which cuts between G and A.