Tag: dna transfer

Questions Related to dna transfer

Transfer of DNA bands from agarose gel to nitrocellulose or nylon membrane is

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Southern transfer

  2. Western transfer

  3. Northern transfer

  4. Eastern transfer

  5. Gene transfer

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Correct Option: A

Gene gun can introduce genes into cells with the help of

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Plasmids

  2. Cosmids

  3. Microscopic pellets

  4. Phagemids

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Correct Option: C
Explanation:

In the gene gun method, microscopic pellets of gold or tungsten are coated with the transgene fragments and shot into the plant cells or tissues at high velocity. 

So the correct option is C.

The technique in which foreign DNA is precipitated over surface of metal particles for passing into target cells is __________.

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Microinjection

  2. Electroporation

  3. Particle gun

  4. Chemical mediated gene transfer

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Correct Option: C

Microparticles for coating with DNA to be bombarded with gene gun are made of

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Silver or platinum

  2. Platinum or zinc

  3. Silicon or platinum

  4. Gold or tungsten

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Correct Option: D

Biolistic gun is suitable for

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Transformation of plant cells

  2. Disarming pathogen vectors

  3. DNA finger printing

  4. Constructing recombinant DNA

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Correct Option: A

Agrobacterium tumefaciens is most widely used for gene transfer because of/ as 

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. It causes crown gall tumours

  2. Its ability to insert Ti plasmid into nuclear genome

  3. It can grow anywhere

  4. It has the ability to kill pathogenic bacteria

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Correct Option: B

Which one of the following is the method of gene transfer based on the use of short electrical impulses of high field strength?

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Ultrasoniation

  2. Electroporation

  3. Microprojectile

  4. Macro injection

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Correct Option: A

The most commonly used method of choice for gene transfer using immature embryos as explants is ______________.

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Microprojectile

  2. Electroporation

  3. Liposome medfeted

  4. Chemically stimulated

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Correct Option: A

Polyethylene glycol method is used for :

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Energy production from sewage

  2. Gene transfer without a vector

  3. Biodiesel production

  4. Seedless fruit production

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Correct Option: B

Electroporation in cloning is

botany option b: biotechnology and bioinformatics dna transfer rdna technology - dna transfer and transformation biotechnology in agriculture

  1. Introduction of genetic material to transgenic animal.

  2. Introduction of genetic material through viruses.

  3. Manipulation of cells by exposing them to a strong electric field.

  4. Injecting DNA into the egg.

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Correct Option: C
Explanation:

Electroporation or electropermeabilization, is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, or DNA to be introduced into the cell. In molecular biology, the process of electroporation is often used to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA.