Tag: botany

Questions Related to botany

$T _1$ plasmid is used for making transgenic plants. It is obtained from

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Azotobacter

  2. Agrobacterium tumefaciens

  3. Rhizobium in leguminous root

  4. Yeast

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Correct Option: B

Plasmids are suitable vectors for gene cloning because they are

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Small circular DNA molecules with their own origin of replication site

  2. Small circular DNA molecules which can integrate with host chromosomal DNA

  3. Having antibiotic genes

  4. Able to shuttle between prokaryotic and eukaryotic cells

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Correct Option: A

Plasmids are used as vectors in genetic engineering because of their

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Resistance to antibiotics

  2. Resistance to restriction enzymes

  3. Ability to carry foreign genes

  4. Ability to cause infection in host

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Correct Option: C

Insect tolerant gene from Bacillus thuringiensis is introduced using $T 1$ plasmid of _______.

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Escherichia coli

  2. Agrobacterium tumefaciens

  3. Haemophilus influenzae

  4. Arabidopsis thaliana

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Correct Option: B

Vector for T-DNA is

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Salmonella typhimurium

  2. Thermits aquaticus

  3. Agrobacterium tumefaciens

  4. Escherichia coli

  5. Bacillus thuringiensis

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Correct Option: C

Which of the following is correctly matched?

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Agrobacterium tumefaciens- Tumour

  2. pBR 322- Enzyme

  3. Thermus aquaticus- Bt gene

  4. Ligase- Molecular scissors

  5. Hind II- Plasmid vector

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Correct Option: A

Pathogenic entity that is responsible for the production of tumour in most dicot plants is

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Retrovirus

  2. Bacteriophage

  3. Ti plasmid

  4. Vector

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Correct Option: C
Explanation:

Agrobacterium is a genus of Gram-negative bacteria that causes tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus. It contains "Ti" plasmid or tumor inducing plasmid, that causes  crown-gall disease in plants. The disease is characterised by a tumour-like growth or gall on the infected plant, often at the junction between the root and the shoot. Tumors are incited by the conjugative transfer of a DNA segment (T-DNA) from the bacterial tumour-inducing (Ti) plasmid to the plant cell naturally when the bacterium infects the plant through  a wound.


So, the correct answer is 'Ti plasmid'

Diseases like malaria and filariasis are transmitted through insect vectors .Vectors can be controlled by introducing fish

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. Labeo

  2. Gambusia

  3. Hilsa

  4. Catla

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Correct Option: B

Consider the following statements:
1. AgrobacteriMm tMmejaciens is a soil bacterium.
2. AgrobacteriMm tumefaciens was the first vector used for introduction offoreign DNA in plant cells.
Which of the statements given above is/are correct?

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. 1 only

  2. 2 only

  3. Both 1 and 2

  4. Neither 1 nor 2

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Correct Option: A

pBR $322$ which is frequently used as a vector for cloning gene in E.coli is?

botany option b: biotechnology and bioinformatics biotechnology in agriculture gm crops transgenic organisms

  1. An original bacterial plasmid

  2. A modified bacterial plasmid

  3. A viral genome

  4. A transposon

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Correct Option: A